铁过量补充对子代大鼠脑铁蓄积及氧化损伤影响

发布时间：2018-01-15 23:07

本文关键词：铁过量补充对子代大鼠脑铁蓄积及氧化损伤影响　出处：《中国公共卫生》2015年02期 　论文类型：期刊论文

【摘要】：目的探讨亲子2代连续铁补充对子代大鼠脑组织铁蓄积及氧化损伤影响。方法 24只4周龄Wistar大鼠(雌:雄=2:1),随机分为4组,分别为对照组、低、中、高剂量铁负荷组(饲料含铁浓度分别为50、150、450、1 350 mg/kg),12周后交配繁殖,按原始组别继续饲喂子代大鼠14周,每组调整子鼠至10只,实验结束时测定脑组织匀浆铁含量、丙二醛、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及血清铁含量。结果低、中、高剂量铁负荷组子鼠脑组织铁含量[分别为(103.05±4.73)、(130.72±34.82)、(134.17±29.45)μg/g]高于对照组[(68.72±6.60)μg/g](P0.05),中、高剂量铁负荷组子鼠血清铁含量[分别为(2.04±0.21)、(2.13±0.21)μg/L]高于对照组[(1.69±0.8)mg/L](P0.05);低、中、高剂量铁负荷组子鼠脑组织MDA含量[分别为(4.41±0.78)、(5.05±0.25)、(4.40±1.16)nmol/mg prot]高于对照组[(3.25±0.71)nmol/mg prot](P0.05);中剂量铁负荷组子鼠脑组织GSH-Px活性[(1.39±0.40)活力单位]明显高于对照组[(0.96±0.35)活力单位](P0.05)。结论亲子2代连续高铁负荷,可使子代成年大鼠血清及脑组织铁含量升高,并可明显增加机体脑组织氧化应激水平。
[Abstract]:Objective to investigate the effects of parent-offspring continuous iron supplementation on iron accumulation and oxidative damage in the brain of offspring rats. Methods 24 4-week-old Wistar rats (female: male 2: 1) were randomly divided into 4 groups. They were control group, low, medium and high dose iron loading group (feed iron concentration was 50 ~ 150 ~ 450 mg 路kg ~ (-1) mg 路kg ~ (-1) ~ (-1) mg / kg), respectively, after 12 weeks mating and reproduction. According to the original group, the rats were fed for 14 weeks with 10 rats in each group. At the end of the experiment, the content of iron, malondialdehyde (MDA) and superoxide dismutase (SOD) in brain homogenate were measured. Glutathione peroxidase (GSH-Px) and serum iron content. [The values of 130.72 卤34.82 渭 g / g were 103.05 卤4.73 渭 g / g, respectively, and 134.17 卤29.45 渭 g / g were higher than those in the control group. [68.72 卤6.60 渭 g / g, P 0.05N, medium and high dose iron load group. [It was 2.04 卤0.21 渭 g / L and 2.13 卤0.21 渭 g / L, respectively, which was higher than that in the control group. [The content of MDA in brain tissue of rats with low, medium and high doses of iron was 1.69 卤0.8 mg / L. [4.41 卤0.78 nmol / mg prot were 4.40 卤1.16 nmol / mg prot, respectively, and 5.05 卤0.25 nmol / mg prot in the control group were higher than those in the control group. [GSH-Px activity of brain tissue in medium dose iron loading group was 3.25 卤0.71 nmol / mg prot. [The activity unit of 1.39 卤0.40) was significantly higher than that of the control group. [Conclusion the iron content in serum and brain tissue of adult rats can be increased by continuous high iron load of parent-offspring generation 2. And can obviously increase the level of oxidative stress in brain tissue.
【作者单位】：青岛大学医学院医学营养研究所;
【基金】：国家自然科学基金(81373000)
【分类号】：R114
【正文快照】： 铁是人体必需营养元素之一,广泛参与机体生理功能和生化反应。铁作为催化剂在氧化还原反应中进行电子交换,这既是机体许多生命活动基础,也是铁毒性作用基础[1]。机体内的游离铁能与H2O2反应生成具有高度反应活性的羟自由基,诱导氧化应激,从而加剧细胞毒性作用,导致组织损伤[2]

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