高效液相色谱-氢化物发生-原子荧光联用技术测定饮用水中四种形态砷

发布时间：2018-01-16 14:26

本文关键词：高效液相色谱-氢化物发生-原子荧光联用技术测定饮用水中四种形态砷　出处：《环境与职业医学》2017年04期 　论文类型：期刊论文

【摘要】：[目的]建立高效液相色谱-氢化物发生-原子荧光(HPLC-HG-AFS)联用技术测定生活饮用水中四种形态砷的方法。[方法]通过高效液相色谱实现水中形态砷的分离,原子荧光进行定性及定量。对硼氢化钾质量分数、盐酸体积分数、氢氧化钠质量分数、缓冲液质量浓度和溶液p H值等实验条件进行优化,再运用到实际样本检测中。[结果]三价砷[As(Ш)]、一甲基砷酸(MMA)、二甲基砷酸(DMA)和五价砷[As(V)]的检出限分别为0.36、0.69、0.39和2.34μg/L,四种形态砷的标准曲线相关系数均大于0.999,平均加标回收率为95.7%~107.9%,相对标准偏差为0.23%~9.31%。[结论]采用HPLC-HG-AFS技术测定饮用水中不同形态砷,检出限低,精密度好,结果准确可靠。
[Abstract]:[Objective to establish a high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS) method for the determination of four forms of arsenic in drinking water. [Methods] the speciation of arsenic in water was separated by HPLC. The mass fraction of potassium borohydride, hydrochloric acid and sodium hydroxide were determined qualitatively and quantitatively by atomic fluorescence spectrometry. The experimental conditions such as buffer concentration and pH value of the solution were optimized and applied to the actual sample detection. [Results] trivalent arsenic. [As( +), monomethyl arsenate (MMAA), dimethyl arsenate (DMA) and pentavalent arsenic (as). [The detection limits were 0.36 渭 g / L and 2.34 渭 g / L, respectively. The correlation coefficients of the standard curves of the four forms of arsenic were all greater than 0.999. The average recovery was 95.7% and the relative standard deviation was 0.233.31. [Conclusion: the determination of arsenic in drinking water by HPLC-HG-AFS has the advantages of low detection limit, good precision, and accurate and reliable results.
【作者单位】：武汉科技大学公共卫生学院职业危害识别与控制湖北省重点实验室;
【分类号】：R123.1
【正文快照】： 砷(As)是一种具有准金属特性的非金属元素,最早以砒霜为人们所熟知,在环境中普遍存在[1]。自然水域的砷污染是一个世界性问题[2]。水中砷污染的来源主要有:自然源,包括矿物及岩石的风化、火山的喷发和温泉上溢;人工源,主要是砷化物的开采和冶炼[3]。上述因素对地下水及饮用水

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