化学消毒的中和剂对水中内毒素活性检测的影响

发布时间：2018-01-16 15:38

本文关键词：化学消毒的中和剂对水中内毒素活性检测的影响　出处：《环境科学》2015年05期 　论文类型：期刊论文

【摘要】：本研究选取化学消毒的常用6种中和剂组氨酸、甘氨酸、抗坏血酸、吐温80、亚硫酸钠和硫代硫酸钠,采用动态浊度鲎试验定量检测样品中内毒素活性,研究中和剂在不同浓度范围内单独使用对内毒素活性检测的影响,旨在优选出适于鲎法检测细菌内毒素的中和剂种类,确定合适的浓度范围.结果表明在0~1.0%浓度范围内,除了甘氨酸和硫代硫酸钠之外,组氨酸、抗坏血酸、吐温80、亚硫酸钠(碱性和中性)均对鲎试验结果有不同程度的干扰,都不能在鲎试验之前作为化学消毒的中和剂.虽然0~1.0%浓度的甘氨酸对鲎试验结果基本无明显影响,但是甘氨酸和戊二醛的中和产物显黄色,所以不适于在光度法鲎试验中用作戊二醛的中和剂.0~1.0%浓度的硫代硫酸钠对鲎试验结果基本无明显干扰,但是当浓度升高至1.0%~5.0%时会对鲎试验结果有一定的抑制作用.与组氨酸、甘氨酸、抗坏血酸、吐温80和亚硫酸钠相比,硫代硫酸钠更适于在内毒素活性检测之前用作消毒剂的中和剂,但是浓度应控制在0.5%范围以内.
[Abstract]:In this study, six neutralizers, histidine, glycine, ascorbic acid, Tween 80, sodium sulfite and sodium thiosulfate, were selected for chemical disinfection. To study the effect of neutralizing agent in different concentration range on endotoxin activity test, the purpose of this study was to select the suitable neutralizer for bacterial endotoxin detection by Limulus method. The results showed that, in addition to glycine and sodium thiosulfate, histidine, ascorbic acid and tween 80 were found in the range of 1.0%. Sodium sulfite (alkaline and neutral) interfered with the results of Limulus test to varying degrees. The neutralization products of glycine and glutaraldehyde were yellow, although the concentration of 1.0% glycine had no obvious effect on the results of Limulus amebocyte lysate test. Therefore, sodium thiosulfate at the concentration of 1.0% was not suitable to be used as a neutralizer for glutaraldehyde in photometric Limulus test. However, when the concentration increased to 1.0 ~ 5.0, there was a certain inhibitory effect on the results of Limulus test, compared with histidine, glycine, ascorbic acid, Twain 80 and sodium sulfite. Sodium thiosulfate is more suitable as a neutralizer for disinfectants before endotoxin activity testing, but the concentration should be kept within 0.5%.
【作者单位】：军事医学科学院疾病预防控制所;清华大学环境学院;
【基金】：国家自然科学基金项目(51378283,50978142,51008303) 北京市自然科学基金项目(8142033)
【分类号】：R123
【正文快照】： 细菌内毒素又称脂多糖,是革兰氏阴性细菌和某些蓝藻的细胞壁组分物质,活菌释放较少,主要由菌体解体后释放[1].内毒素是常见的外源性致热原,属于强免疫刺激因子,具有广泛的生物活性,与多种人类疾病密切相关.机体对内毒素反应极为敏感,Elin等[2]报道使用0.1~0.5 ng·kg-1内毒素

【参考文献】