L-茶氨酸改善热应激引起的小鼠组织损伤和氧化逆境

发布时间：2018-01-18 08:33

本文关键词：L-茶氨酸改善热应激引起的小鼠组织损伤和氧化逆境　出处：《茶叶科学》2017年01期 　论文类型：期刊论文

【摘要】：以SPF级Slca/KM雄性小鼠作为实验对象,在恒温恒湿模拟气候箱进行热处理,灌喂干预不同剂量L-茶氨酸,考察L-茶氨酸对小鼠采食量、体重变化、空肠切片病理情况、器官指数、血清谷丙转氨酶(ALT)与谷草转氨酶(AST)活性、血清肿瘤坏死因子(TNF-α)、白细胞介素6(IL-6)和干扰素-γ(IFN-γ)含量、肝组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性以及肝组织病理学变化的影响,以探讨L-茶氨酸改善热处理引起的小鼠组织损伤和氧化逆境作用。结果表明,L-茶氨酸能在一定程度上促进热处理小鼠采食量和体重的增加,降低肝脏和脾脏器官指数,降低血清ALT、AST酶活性,抑制血清炎症介质TNF-α、IL-6、IFN-γ的含量,降低肝组织MDA含量,提高SOD、GSH-Px、CAT酶活性,减轻热处理对空肠及肝脏组织的损伤,且以L-茶氨酸中剂量处理效果较好。说明L-茶氨酸具有改善热处理引起的小鼠组织损伤和氧化逆境的作用,主要表现为提高小鼠营养物质吸收能力、减少炎性细胞因子表达,以及降低氧化损伤。
[Abstract]:The male Slca/KM mice of SPF grade were treated by heat treatment in a simulated climate chamber with constant temperature and humidity, and were fed with different doses of L-theanine, and the intake of L-theanine to mice was investigated. Body weight change, pathological condition of jejunum section, organ index, serum alanine aminotransferase (alt) and aspartate aminotransferase (AST) activity, serum tumor necrosis factor TNF- 伪). The contents of IL-6 and IFN- 纬, the content of malondialdehyde (MDAA) in liver tissue and the content of superoxide dismutase (SOD). Effects of glutathione peroxidase (GSH-Px) and catalase (catalase) activity and hepatic histopathology. To study the effects of L-theanine on tissue damage and oxidative stress induced by heat treatment in mice, the results showed that L-theanine could promote the increase of feed intake and body weight of heat-treated mice to some extent. The indexes of liver and spleen organs were decreased, the activity of serum alt AST enzyme was decreased, the content of TNF- 伪 and IL-6 IFN- 纬 was inhibited, and the content of MDA in liver tissue was decreased. The activity of GSH-PxPxcat was increased and the injury of jejunum and liver was alleviated by heat treatment. The results showed that L-theanine could improve the tissue damage and oxidative stress induced by heat treatment in mice, and the main effect was to improve the absorption ability of nutrients in mice. Reduce the expression of inflammatory cytokines, as well as reduce oxidative damage.
【作者单位】：湖南农业大学园艺园林学院茶学教育部重点实验室;湖南农业大学国家植物功能成分利用工程技术研究中心;湖南农业大学湖南省植物功能成分利用协同创新中心;
【基金】：国家“十二五”科技支撑计划项目(2011BAD10B00)
【分类号】：R151
【正文快照】： 热应激是机体对高温应激原所产生的一种非特异性防御应答反应,在热应激条件下,机体须通过调节自身代谢率等途径来减少产热、增加散热,以适应高温环境[1],但当热应激超出机体自我调节范围时,则会导致生理功能紊乱,出现生长与生产性能降低等危害[2]。肝脏是机体内以代谢功能为主

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