妊娠期全氟辛酸染毒对子代雄鼠发育毒性的研究

发布时间：2018-01-18 11:06

本文关键词：妊娠期全氟辛酸染毒对子代雄鼠发育毒性的研究　出处：《环境科学学报》2017年10期 　论文类型：期刊论文

【摘要】：探讨妊娠期染毒全氟辛酸(perfluorooctanoic acid,PFOA)对子代雄鼠发育毒性的影响.将50只妊娠0 d小鼠随机分5组,每组10只,妊娠0~17 d每天按0、1、2.5、5和12.5 mg·kg~(-1)BW饲喂PFOA,产后仔鼠正常饲养,记录仔鼠存活数.产后21 d,检测仔鼠血清睾酮水平并计算睾丸指数;HE染色观察睾丸组织结构变化;RT-PCR法检测睾丸组织Dlk1-Dio3印记基因簇各目的基因mRNA的表达情况.结果显示,随PFOA染毒剂量增加,仔鼠存活数显著降低,血清睾酮含量极显著性降低(p0.01);睾丸指数无统计学意义;睾丸组织有不同程度的损伤并呈剂量依赖性;Dlk1-Dio3印记基因簇各目的基因的表达量都有降低趋势,其中Gtl2、Rian、Dio3均显著性降低(p0.05).结果表明,妊娠期染毒PFOA能够降低仔鼠存活数,损伤睾丸组织结构,并能够扰乱生殖激素,降低睾丸组织Dlk1-Dio3印记基因簇各目的基因mRNA的表达.
[Abstract]:To investigate the effects of perfluorooctanoic acidoic acid (PFOAA) on developmental toxicity of male offspring during pregnancy, 50 mice were randomly divided into 5 groups. 10 rats in each group were fed with 0.5 mg 路kg~(-1)BW and 12.5 mg 路kg~(-1)BW on the 17th day of gestation, and puerperal mice were fed normally. After 21 days postpartum, the serum testosterone level and testicular index were measured. The structure of testis was observed by HE staining. RT-PCR method was used to detect the mRNA expression of Dlk1-Dio3 imprinted gene cluster in testis. The results showed that the survival number of mice decreased significantly with the increase of PFOA dose. The serum testosterone level was significantly lower than that of the control group (P 0.01). Testicular index had no statistical significance. Testicular tissue was damaged in a dose-dependent manner. The expression of all target genes in Dlk1-Dio3 imprinted gene cluster showed a tendency to decrease, and the expression of Gtl2 + Rianli Dio3 decreased significantly (P 0.05). Exposure to PFOA during pregnancy can reduce the number of live offspring, damage the structure of testis, and disrupt reproductive hormones. Reduce the expression of mRNA of Dlk1-Dio3 imprinting gene cluster in testis.
【作者单位】：河北农业大学中兽医学院;河北农业大学动物科技学院;
【基金】：国家自然科学基金(No.31502111) 河北省自然科学基金(No.C2016204097)~~
【分类号】：R114
【正文快照】： 1引言(Introduction)全氟辛酸(Perfluorooctanoic acid,PFOA)是全氟化合物(Perfluoroalkyl substances,PFASs)中最具有代表性化合物之一,作为一种新型持久性有机污染物,被广泛地应用于工业生产和人们的日常生活中,在全球范围内的各种环境介质中均被检测到.由于PFOA半衰期较长

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