镉对肾小管上皮细胞中原癌基因c-myc表达的影响

发布时间：2018-01-22 02:39

本文关键词： 镉肾小管上皮细胞 c-myc基因　出处：《环境与健康杂志》2017年08期 　论文类型：期刊论文

【摘要】：目的探讨镉对肾小管上皮细胞(TEC)毒效应及原癌基因c-myc表达的影响。方法将处于对数生长期的TEC分别暴露于含终浓度为0(对照)、0.625、1.25、2.50、5.0、10.0μmol/L氯化镉的培养基染毒24、48、72 h后,采用MTT法测定TEC的存活情况。将处于对数生长期的TEC分别暴露于含终浓度为0(对照)、0.625、1.25、2.50μmol/L氯化镉的培养基染毒24、48、72 h后,采用RT-qPCR技术检测TEC c-myc mRNA的表达水平。结果与对照组比较,2.50、5.0μmol/L氯化镉染毒24、48、72 h的TEC存活率较高,除5.0μmol/L氯化镉染毒72 h外,差异均有统计学意义(P0.05)。随着氯化镉染毒剂量的升高,不同染毒时间TEC的存活率均呈先上升后下降的趋势,且均以2.50μmol/L组最高;随着氯化镉染毒时间的延长,2.50、5.0、10.0μmol/L组TEC的存活率均呈下降趋势。与对照组比较,各剂量氯化镉染毒不同时间后TEC中c-myc mRNA的表达水平均较高,差异有统计学意义(P0.05);且随着氯化镉染毒剂量的升高,不同染毒时间TEC中c-myc mRNA的表达水平均呈上升趋势。结论一定低剂量镉诱导TEC中c-myc持续激活和过表达与镉诱导TEC过度增殖密切相关,可能是镉致肾损伤的毒作用机制之一。
[Abstract]:Objective to investigate the effects of cadmium on the toxic effects of cadmium on tubular epithelial cells (TECs) and the expression of proto-oncogene c-myc. Methods TEC in logarithmic growth phase was exposed to a final concentration of 0 (control). The culture medium of 0.625 ~ 1.25 ~ 1.25 ~ 2.50 ~ 5.0 渭 mol/L cadmium chloride was inoculated with cadmium chloride for 24 ~ 4 ~ 872 h. The survival of TEC was measured by MTT method. The TEC in logarithmic growth phase was exposed to 0 (control group) 0.625 ~ 1.25 respectively. The culture medium containing 2.50 渭 mol/L cadmium chloride was inoculated with 24 渭 mol/L for 48 minutes for 72 hours. The expression of TEC c-myc mRNA was detected by RT-qPCR technique. The survival rate of TEC was higher at 72 h, except 5.0 渭 mol/L cadmium chloride for 72 h, the difference was statistically significant (P 0.05). The survival rate of TEC increased first and then decreased at different exposure time, and the highest rate was in 2.50 渭 mol/L group. With the prolongation of cadmium chloride exposure time, the survival rate of TEC decreased in the group of 2.50 渭 mol/L, 5.0 渭 mol/L and 10.0 渭 mol / L, compared with the control group. The expression of c-myc mRNA in TEC was significantly higher than that in control group (P 0.05). And with the increase of cadmium chloride exposure dose. C-myc in TEC with different exposure time. Conclusion the continuous activation and overexpression of c-myc in TEC induced by certain low dose cadmium is closely related to the excessive proliferation of TEC induced by cadmium. It may be one of the toxic mechanisms of renal injury induced by cadmium.
【作者单位】：广东药科大学公共卫生学院;
【基金】：2014年广东高校国家级大学生创新训练计划(201410573005) 深圳市科技计划(JCYJ20140414154847275) 广东省预防医学应用型人才培养模式创新实验区(粤财教[2011]473号)
【分类号】：R114
【正文快照】： 镉(cadmium,Cd)是常见的职业和环境毒害物,被国际癌症研究机构(IARC)确定为Ⅰ类致癌物,是人和实验动物罹患肺癌和前列腺癌的确认致癌物[1]。肾是镉毒性最敏感的的靶器官,肾功能异常是镉暴露的临界效应[2]。目前,大多数研究普遍认为一定剂量(20μmol/L)Cd可抑制肾脏细胞正常增

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