直链烷基苯磺酸钠对小鼠皮肤组织老化的促进作用

发布时间：2018-01-24 10:56

本文关键词： 直链烷基苯磺酸钠皮肤组织老化　出处：《中国老年学杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的探讨直链烷基苯磺酸钠(LAS)对小鼠皮肤组织衰老的影响。方法将50只健康昆明种雄性小鼠,随机分成5组,每组10只。各组分别为正常对照组、LAS低、中、高剂量组(LAS 150、300、600 mg/L)及D-半乳糖衰老模型组。正常对照组及LAS各剂量组分别用蒸馏水及不同剂量的LAS涂抹小鼠的背部剃毛处,连续涂抹60 d,衰老模型组小鼠每日在颈背部皮下注射5%D-半乳糖,连续注射60 d。取涂抹部位皮肤进行Masson及β-半乳糖苷酶染色;检测皮肤匀浆中丙二醛(MDA)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性;测定皮肤组织中羟脯氨酸(Hyp)含量。结果与正常对照组相比,LAS各剂量组及衰老模型组小鼠皮肤组织变薄,胶原纤维减少,纤维排列杂乱、松散、模糊;β-半乳糖苷酶表达程度明显增高;MDA含量明显增加(P0.01),SOD、GSH-Px活性及Hyp含量均明显降低(P0.01)。结论 LAS可促进小鼠皮肤组织的老化。
[Abstract]:Objective to investigate the effect of sodium alkylbenzenesulfonate (LAS) on skin senescence in mice. Methods 50 healthy male Kunming mice were randomly divided into 5 groups with 10 in each group. Low, medium and high dose LAS groups were treated with Las 150,300. Normal control group and LAS group were smeared with distilled water and different doses of LAS for 60 days. Mice in aging model group were injected with 5 Dgalactose subcutaneously daily and continuously for 60 days. The skin was smeared for Masson and 尾 -galactosidase staining. The content of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in skin homogenate were determined. Results compared with the normal control group and the aging model group, the skin tissue became thinner, the collagen fiber decreased, the fiber arrangement was disorganized and loose. Fuzzy; The expression of 尾 -galactosidase was significantly increased. The content of MDA significantly increased the activity of GSH-Px and the content of Hyp decreased significantly. Conclusion LAS can promote the aging of skin tissue of mice.
【作者单位】：蚌埠医学院预防医学系;
【基金】：安徽省高等学校省级自然科学研究重点项目(KJ2014A159) 安徽省高等学校预防医学特色专业基金资助项目(皖教高〔2011〕5号)
【分类号】：R114
【正文快照】： 直链烷基苯磺酸钠(LAS)是目前最普遍使用的阴离子型表面活性剂,是家用洗涤用品中的主要去污成分〔1〕。LAS对人属低毒类物质,难以降解,但在生产与生活过程中长期接触可使机体产生复杂的综合性的不良反应,尤其是容易残留于水中,或在生产或生活过程中易于与人的皮肤接触。有报道

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