PAS-Na对染锰大鼠丘脑和基底节氨基酸类神经递质及铁代谢的影响

发布时间：2018-01-24 21:19

本文关键词： 对氨基水杨酸钠锰氨基酸类神经递质铁蛋白转铁蛋白　出处：《广西医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：[目的]探讨PAS-Na对锰致大鼠丘脑和基底节氨基酸类神经递质水平和铁代谢的影响。[方法]130只雄性SD大鼠按完全随机法分为染锰组、PAS-Na预防性干预组(P-PAS组)、低剂量PAS-Na (L-PAS)治疗组、中剂量PAS-Na (M-PAS)治疗组、高剂量PAS-Na (H-PAS)治疗组和正常对照(对照组),观察期为4周(染锰4周)、8周(染锰+PAS预防8周)、12周(染锰+PAS预防12周)、18周(染锰12周+治疗6周)。给染锰、预防、L-、M-和H-PAS治疗组腹腔注射MnCl2·4H2O15mg/kg,对照组腹腔注射等容量生理盐水,每日1次,每周5d,连续4周、8周或12周。P-PAS组染锰的同时背部皮下注射PAS-Na200mg/kg,每周3次,连续8周或12周。然后,给L-、M-和H-PAS治疗组背部皮下注射PAS-Na100、200或300mg/kg,其余组背部皮下注射等容量生理盐水,每日1次,连续6周。每周测量一次体重。用HPLC荧光检测法测定丘脑中Glu、Gln、Gly、GABA含量；用ELISA试剂盒测定丘脑和基底核中铁蛋白、转铁蛋白含量。[结果]观察期2、3、4、5、8、11周时,染锰组体重都低于对照组(P0.05),P-PAS组体重均高于染锰组,但无显著性差异(P0.05)；观察期18周时,染锰组体重在停止染锰后逐渐恢复至对照组水平,各治疗组大鼠经PAS-Na治疗后体重增长速度逐渐恢复最后接近对照组水平,但各组体重无显著性差异(P0.05)。观察期4、8、12周时,各组大鼠丘脑Glu、Gln、Gly、GABA含量无明显差异(P0.05)。观察期18周时,染锰组Glu水平明显低于对照组,L-PAS治疗组Glu含量明显高于染锰组(P0.05)。观察期4、8、12周时各组丘脑铁蛋白和转铁蛋白含量无显著性差异(P0.05)。观察期18周时,染锰组转铁蛋白含量高于对照组(P0.05),各治疗组转铁蛋白含量较染锰组下降(P0.05),且高PAS治疗组较其余两个剂量治疗组下降明显。在基底节,观察期4、8、12周时各组铁蛋白和转铁蛋白含量无明显差异。观察期18周时,L-PAS治疗组转铁蛋白含量明显低于染锰组(P0.05)。[结论]1.染锰会降低大鼠体重增长速度；2.锰对大鼠丘脑Glu和Gln的损害呈持续性,PAS-Na对染锰大鼠Glu含量的有害影响可能有一定的拮抗作用。3. PAS-Na治疗可能使染锰大鼠丘脑和基底神经节转铁蛋白含量减少。
[Abstract]:[Objective] to investigate the effect of PAS-Na on the level of amino acid neurotransmitters and iron metabolism in rat thalamus and basal ganglia. [Methods: 130 male Sprague-Dawley rats were randomly divided into two groups: PAS-Na preventive intervention group and low-dose PAS-Na L-PAS-treated group. Middle dose PAS-Na M-PAS-treated group, high-dose PAS-Na H-PAS-treated group and normal control group (control group, the observation period was 4 weeks (manganese exposure 4 weeks). 8 weeks (8 weeks after exposure to manganese PAS) and 12 weeks after exposure to manganese PAS (12 weeks after exposure to manganese PAS) and 18 weeks after treatment (12 weeks of manganese exposure and 6 weeks of treatment). M- and H-PAS treatment group were intraperitoneally injected with MnCl2 路4H2O 15mg / kg, while the control group was intraperitoneally injected with normal saline of the same volume, once a day, 5 days a week for 4 weeks. PAS-Na 200 mg / kg was injected subcutaneously into the back of the group of 8 or 12 weeks. Then, L- was given to L-, three times a week for 8 or 12 weeks. In the M- and H-PAS treatment group, PAS-Na 100 mg / kg was injected subcutaneously into the back of the rats, and the rest group was subcutaneously injected with the same volume of normal saline once a day. The body weight was measured once a week for 6 weeks. The content of Glun Gln GlyGABAs in the thalamus was determined by HPLC fluorescence assay. The contents of ferritin and transferrin in thalamus and basal nucleus were determined by ELISA kit. [Results: at 11 weeks after observation, the body weight of the manganese exposed group was lower than that of the control group (P 0.05) and P-PAS group was higher than that of the manganese group, but there was no significant difference (P 0.05). After 18 weeks of observation, the weight of the manganese exposed group gradually recovered to the level of the control group after stopping the exposure to manganese, and the weight growth rate of the rats in each treatment group gradually recovered to the level of the control group after PAS-Na treatment. However, there was no significant difference in body weight in each group (P 0.05). There was no significant difference in GABA content between the two groups. At 18 weeks of observation, the level of Glu in the manganese exposed group was significantly lower than that in the control group. The content of Glu in L-PAS treatment group was significantly higher than that in manganese exposed group (P 0.05). There was no significant difference in the levels of thalamic ferritin and transferrin in each group at 12 weeks. At 18 weeks after observation, the transferrin content in manganese exposed group was higher than that in control group (P 0.05). The levels of transferrin in each treatment group were significantly lower than those in the manganese exposed group, and the levels of transferrin in the high PAS group were significantly lower than those in the other two dose groups. There was no significant difference in the levels of ferritin and transferrin in each group at 12 weeks, but the transferrin content in L-PAS treatment group was significantly lower than that in manganese exposed group at 18 weeks. [Conclusion: 1. Manganese exposure can decrease the weight growth rate of rats; 2. The damage of manganese to Glu and Gln in rat thalamus was sustained. The harmful effect of PAS-Na on Glu content in manganese exposed rats may have some antagonistic effect. 3. The level of transferrin in thalamus and basal ganglion of rats exposed to manganese may be decreased by PAS-Na treatment.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R114

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