西安市采暖季与非采暖季PM2.5染毒的人胚肺细胞基因差异表达研究

发布时间：2018-01-24 22:09

本文关键词： PM. WI-人胚肺细胞基因差异表达斑点杂交 TNF-α IL- IL- IL-　出处：《西安交通大学学报(医学版)》2015年06期 　论文类型：期刊论文

【摘要】：目的寻找与大气污染致病相关的相关基因,为阐明大气污染致病的生物学机制提供科学依据。方法采用mRNA斑点杂交鉴定技术,克隆经采暖季≥75μg/m3 PM2.5与非采暖季75μg/m3 PM2.5染毒的WI-38人胚肺细胞,分析其间的基因表达差异。放免法测定炎性因子TNF-α、IL-2、IL-6和IL-8。结果与对照组比较,PM2.5100μg/mL染毒24h后,WI-38人胚肺细胞细胞因子TNF-α、IL-6和IL-8明显升高,IL-2明显减低(P0.05)。不同浓度PM2.5处理的WI-38人胚肺细胞间差异表达的基因片段,可见48份基因样本在350bp处出现清晰条带;该48份基因样本经斑点杂交后,Tester cDNA杂交的48个斑点中,41份可见黑褐色斑点,而同样样本与Driver cDNA杂交的该48份样本均未见明显显色。结论 PM2.5能诱导WI-38人胚肺细胞产生炎性损伤,≥75μg/m3 PM2.5染毒的WI-38人胚肺细胞存在明显的基因损伤。
[Abstract]:Objective to search for the related genes related to the pathogenesis of atmospheric pollution and to provide scientific basis for elucidating the biological mechanism of the pathogenicity of atmospheric pollution. Methods mRNA dot blot hybridization technique was used to identify the pathogenicity of atmospheric pollution. The lung cells of WI-38 embryos were cloned from 75 渭 g / m3 PM2.5 and 75 渭 g / m3 PM2.5 in heating season and non-heating season respectively. The difference of gene expression between them was analyzed. The levels of IL-6 and IL-8 were measured by radioimmunoassay. The results were compared with those of the control group. After 24 hours of exposure to PM2.5100 渭 g / mL, WI-38 human embryonic lung cell cytokines TNF- 伪, IL-6 and IL-8 were significantly increased. IL-2 significantly reduced the differentially expressed gene fragments of WI-38 human embryonic lung cells treated with different concentrations of PM2.5. 48 samples showed clear bands at 350 BP. After dot blot hybridization, 41 of the 48 spots of 48 gene samples by Tester cDNA hybridization showed dark brown spots. However, none of the 48 samples hybridized with Driver cDNA showed obvious color. Conclusion PM2.5 can induce inflammatory injury in WI-38 human embryonic lung cells. There was significant gene damage in WI-38 human embryonic lung cells exposed to 75 渭 g / m ~ 3 PM2.5.
【作者单位】：西安交通大学医学部;
【基金】：2013年国家级大学生创新训练项目(No.201310698066)~~
【分类号】：R122
【正文快照】： 西安市采暖季PM2.5污染浓度显著高于非采暖季;PM2.5的污染加剧是西安市居民心血管系统和呼吸系统疾病的死亡率升高的原因之一。随着我国大气污染的日趋严重,雾霾范围和程度日益加重,对PM2.5与疾病的发病率、死亡率关系研究,尤其是呼吸系统疾病及心血管疾病发病机制的研究越来

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