邻苯二甲酸二丁酯对成年大鼠睾丸Leydig细胞中Cx43蛋白表达及睾酮分泌的影响

发布时间：2018-01-31 11:25

本文关键词： 邻苯二甲酸二丁酯 Leydig细胞 Cx 睾酮　出处：《吉林大学学报(医学版)》2015年02期 　论文类型：期刊论文

【摘要】：目的:观察邻苯二甲酸二丁酯(DBP)对成年大鼠睾丸Leydig细胞中连接蛋白43(Cx43)的表达及睾酮分泌的影响,探讨DBP对睾酮的调节是否与Cx43有关联。方法:将SD大鼠无菌脱臼处死,原代培养纯化Leydig细胞,将细胞分为溶剂对照组(0.1%DMSO)、DBP组(50 mg·L-1 DBP)、Cx43增强剂组(50mg·L-1 DBP+10μmol·L-1 PGE2)和特异睾酮阻断剂组(40μmol·L-1氟他胺),分别处理细胞24h;放射免疫法测定Leydig细胞睾酮水平;细胞免疫荧光和Western blotting法检测细胞中Cx43蛋白表达。结果:细胞染毒24h后,与DMSO组比较,DBP组Leydig细胞中Cx43蛋白表达水平和睾酮水平均降低(P0.05);与DBP组比较,DBP+PGE2组Leydig细胞中Cx43表达水平升高(P0.05),但睾酮水平变化不明显(P0.05);与DMSO组比较,氟他胺组Leydig细胞中睾酮水平和Cx43蛋白表达水平均明显降低(P0.05)。结论:DBP对睾酮分泌及Cx43表达均有影响,Cx43对睾酮合成无抑制作用,而睾酮可反向调节Cx43表达;DBP对Leydig细胞睾酮合成抑制作用并不一定以Cx43为靶点。
[Abstract]:Objective: to observe the effect of dibutyl phthalate (DBP) on the expression of connexin 43 (Cx43) and testosterone secretion in adult rat testicular Leydig cells. To investigate whether the regulation of testosterone by DBP is related to Cx43. Methods: SD rats were killed in aseptic dislocations and purified Leydig cells in primary culture. Cells were divided into solvent control group (0.1 mg 路L ~ (-1)) and DBP group (50 mg 路L ~ (-1) DBP). Cx43 enhancer group (50 mg 路L -1 DBP 10 渭 mol 路L -1 PGE2) and specific testosterone blocker group (40 渭 mol 路L -1 flutamide). The cells were treated for 24 h; The levels of testosterone in Leydig cells were measured by radioimmunoassay. Results: the expression of Cx43 protein was detected by cell immunofluorescence and Western blotting assay. Results: the expression of Cx43 protein in the cells was compared with that in the DMSO group after 24 hours of exposure. In DBP group, the expression of Cx43 protein and testosterone in Leydig cells decreased significantly (P 0.05). Compared with the DBP group, the expression of Cx43 in Leydig cells in the PGE2 group was higher than that in the control group (P 0.05), but the level of testosterone did not change significantly (P 0.05). Compared with DMSO group. The level of testosterone and the expression of Cx43 protein in Leydig cells in flutamide group were significantly lower than those in control group (P 0.05). Cx43 had no inhibitory effect on testosterone synthesis, while testosterone could reverse regulate the expression of Cx43. The inhibitory effect of DBP on testosterone synthesis in Leydig cells is not necessarily targeted at Cx43.
【作者单位】：北华大学公共卫生学院环境卫生学教研室;
【基金】：吉林省科技厅科技发展计划项目资助课题(20100139,201205038) 吉林省教育厅科研项目资助课题(2011129,2014211) 北华大学大学生国家级创新项目资助课题(201310201050)
【分类号】：R114
【正文快照】： 邻苯二甲酸二丁酯(dibutyl phthalate,DBP)是环境内分泌干扰物中重要的一类,被广泛应用到工业生产中。由于DBP并非以化学键的形式绑定在聚合基质中,因此在其使用过程中不断的向外界环境迁移,造成环境污染[1]。很多研究[2-3]表明:DBP具有显著的雄性生殖毒性,可引起雄性啮齿类动

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