交通性污染对雄性小鼠生殖毒性以及分子机制的初步研究

发布时间：2018-01-31 14:07

本文关键词： 交通污染机动车尾气颗粒物病理组织学单细胞凝胶电泳细胞色素P450侧链裂解酶实时荧光定量RT-PCR　出处：《福建医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：本研究通过建立现场暴露交通污染环境和腹腔注射机动车尾气颗粒物两种染毒试验，观察和分析交通性污染对雄性小鼠生殖毒性作用及可能的分子机制，为人群生育健康的促进和制定有效保护措施提供科学依据。方法：本研究通过建立现场暴露交通污染环境染毒试验（将30只清洁级雄性昆明系小鼠随机分为重度污染暴露组、轻度污染暴露组和清洁环境空白对照组3个组，每组10只，进行现场暴露染毒，每日持续暴露10h，持续暴露35d。）的基础上，结合腹腔注射机动车尾气颗粒物染毒试验（将40只清洁级ICR雄性小鼠随机分为对照组（PBS溶液）、低剂量染毒组(0.4mg/ml)、中剂量染毒组(2mg/ml)和高剂量染毒组(10mg/ml)4个组，每组10只，每只小鼠均按10ml/kg的注射量进行，隔24h注射一次，持续35d）。染毒后观察和分析交通性污染对各组实验小鼠生殖器官发育、精子数量和质量、睾丸和附睾组织病理学变化等可能存在的生殖毒性并通过分析实验小鼠睾丸细胞DNA损伤情况和血清睾酮含量以及与其分泌密切相关基因P450scc和StAR的mRNA的相对表达量探讨可能的分子机制。结果：现场暴露交通污染环境染毒试验和腹腔注射机动车尾气颗粒物染毒试验均发现交通性污染物能影响实验小鼠生殖器官的发育，现场暴露交通污染环境染毒试验的脏器系数分析显示与对照组小鼠相比，重度暴露组小鼠体重、睾丸和附睾重、睾丸脏器系数下降；腹腔注射机动车尾气颗粒物染毒试验显示高剂量组的附睾质量、附睾脏器系数下降，差异有统计学意义(P＜0.05)。组织病理学结果显示：现场暴露交通污染环境染毒试验中轻度和重度暴露组以及腹腔注射机动车尾气颗粒物染毒试验中中剂量和高剂量小鼠睾丸和附睾组织有不同程度损伤。与对照组相比，轻度和重度暴露组以及中剂量和高剂量小鼠精子数量下降，精子畸形率上升，经检验以上差异均具有统计学意义（P0.05）；在分子机制研究中彗星试验显示：重度暴露组小鼠睾丸细胞DNA损伤率增加，与空白对照组相比，差异具有统计学意义（P0.05）；腹腔注射机动车尾气颗粒物染毒试验显示高剂量组血清睾酮含量以及睾丸组织基因P450scc mRNA相对表达量，，与对照组相比下降,差异具有统计学意义(P＜0.05)。结论：交通污染以及交通污染重要组成成分机动车尾气颗粒物可能通过直接损睾丸细胞DNA和（或）影响睾丸的分泌以及与其分泌密切相关的基因P450sccmRNA的相对表达量的作用机制对雄性小鼠生殖系统的发育和功能造成一定程度的损伤。
[Abstract]:Objective: to observe and analyze the effect of traffic pollution on male mice reproductive toxicity and its possible molecular mechanism by setting up two kinds of exposure tests: field exposure to traffic pollution environment and intraperitoneal injection of motor vehicle exhaust particulates to observe and analyze the effects of traffic pollution on male mice reproductive toxicity. To provide scientific basis for the promotion of population reproductive health and the formulation of effective protection measures. Methods: in this study, 30 clean grade male Kunming mice were randomly divided into severe pollution exposure group by setting up field exposure test. Light pollution exposure group and clean environment blank control group 3 groups, each group 10 rats, field exposure exposure, 10 hours a day, continuous exposure 35 d.on the basis of. Combined with intraperitoneal injection of motor vehicle exhaust particles (40 clean grade ICR male mice were randomly divided into control group, low dose group, 0. 4 mg / ml). The middle dose group (2 mg / ml) and the high dose group (10 mg / ml), 10 mice in each group were injected at 10 ml / kg, once every 24 hours. After 35 days of exposure, the development of reproductive organs, the quantity and quality of spermatozoa were observed and analyzed. The possible reproductive toxicity of testicular and epididymal histopathological changes was analyzed by analyzing the DNA damage in testicular cells, serum testosterone content and the genes P450 SCC and S closely related to the secretion of testicular cells in experimental mice. The molecular mechanism of the relative expression of mRNA in tAR was studied. Results: it was found that traffic pollutants could affect the development of reproductive organs of experimental mice in field exposure to traffic pollution environment and intraperitoneal injection of motor vehicle exhaust particulates. Compared with the control group, the body weight, testis and epididymis weight and testis organ coefficient in the severe exposure group were lower than those in the control group. The mass of epididymis and organ coefficient of epididymis were decreased in high dose group. The difference was statistically significant (P < 0.05). ... histopathological results show that:. The testis and epididymis tissues of mice exposed to traffic pollution in the mild and severe exposure group and the intraperitoneal injection of motor vehicle exhaust particulates were damaged in varying degrees, as compared with those in the control group. Compared to. In mild and severe exposure groups, the number of sperm decreased and the rate of sperm malformation increased in moderate and high dose mice. The above differences were statistically significant (P 0.05). In the study of molecular mechanism, comet assay showed that the DNA damage rate of testicular cells in the severe exposure group was increased, compared with the control group, the difference was statistically significant (P 0.05). The experimental results of intraperitoneal injection of motor vehicle exhaust particles showed that the serum testosterone content and the relative expression of P450 SCC mRNA in testis tissue in high dose group were lower than those in control group. The difference was statistically significant (P < 0.05). Conclusion: traffic pollution and traffic pollution are important components of vehicle exhaust particles, which may directly damage testicular cells DNA and (or). The mechanism of affecting the secretion of testis and the relative expression of P450scc mRNA, which is closely related to the secretion of testis, caused some damage to the development and function of the reproductive system of male mice.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R114

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