黑龙江地区典型人群酸菜中亚硝酸盐暴露的调查研究

发布时间：2018-01-31 18:36

本文关键词： 亚硝酸盐氧化应激 SOD 细胞因子流式细胞术　出处：《宁夏医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:通过调查黑龙江地区酸菜摄入水平及酸菜、泡菜中亚硝酸盐的含量,了解该地区人群膳食酸菜、泡菜摄入亚硝酸盐的情况,并进一步探讨亚硝酸盐摄入与人体产生氧化应激的关系。用流式细胞术检测膳食酸菜及泡菜摄入亚硝酸盐人群血浆中IL-17A、IFN-γ、TNF、IL-10、IL-6、IL-4、IL-2等细胞因子以及趋化因子CXCL10水平,探讨膳食酸菜及泡菜而摄入亚硝酸盐水平与人体炎性因子的关系。方法:通过黑龙江牡丹江市西安区海南乡两个村的典型人群调查,选择80人作为调查对象,分别在12月初和2月底收集对象的一天的膳食酸菜、泡菜摄入样本,进行一般人口学调查,并收集两次的血液样本。通过GB 5009.33—2010测定膳食酸菜、泡菜中亚硝酸盐的含量,结合膳食酸菜及泡菜的摄入计算膳食亚硝酸盐的摄入量;利用比色法测定调查对象血浆中的亚硝酸盐含量;利用比色法测定血浆中CAT、SOD的含量;采用流式细胞细胞术(CBA)检测各组血浆中IL-17A、IFN-γ、TNF、IL-10、IL-4、IL-2等细胞因子水平,采用ELISA双抗体夹心法检测血浆中CXCL10水平。根据这种摄入的四分位数间距进行分组,以下四分位数间距作为参比组(对照组),第1-4组分别为20、20、20、20。检测并比较高摄入组与对照组人体血浆中CAT、SOD的活性水平。比较高摄入与对照组人体血浆中IL-17A、IFN-γ、TNF、IL-10、IL-4、IL-2等细胞因子及趋化因子CXCL10水平。结果:描述调查地区典型人群的一般人口学特征,第一次调查膳食酸菜摄入为195.98±79.32g/天/人,第二次调查膳食酸菜摄入为178.89±49.55g/天/人。第一次收集到70份酸菜样品,第二次收集到66份酸菜样品。共检测136份样品中亚硝酸盐的检出率为70.6%,超标率为0.7%。第一次调查血浆样品中亚硝酸盐的平均浓度为13.79±11.27 uM,第二次调查收集到的80份血浆样本中亚硝酸盐的平均浓度为11.67±6.31 uM。以调查对象亚硝酸盐摄入四分位间距进行分组,1-4组分别亚硝酸盐摄入分别为:82.98±75.86ug、273.77±33.81ug、405.27±39.63ug、723.33±147.95ug。膳食酸菜及泡菜后,人体血浆中亚硝酸盐含量基本稳定。膳食酸菜及泡菜后,人体血浆中中CAT活力、CXCL10水平趋于稳定,SOD活力有一定的抑制作用。膳食酸菜及泡菜后人体血浆中IL-17A、IFN-γ、TNF、IL-10、IL-6、IL-4等细胞因子水平有所下降。结论:被调查地区酸菜、泡菜摄入量调查显示,典型人群膳食酸菜及泡菜中亚硝酸盐在国家标准限定的范围内。不同酸菜消费者血液含量变化无差别。膳食酸菜及泡菜摄入亚硝酸盐后,对SOD活力有一定的抑制作用。本试验研究膳食不同酸菜消费者体内炎性因子发生变化与亚硝酸盐的摄入量存在一定相关性,随着膳食酸菜及泡菜中亚硝酸盐总摄入水平的增高,人体血浆中IL-17A、TNF、IL-10、IL-6、IL-4等细胞因子水平有所下降。
[Abstract]:Objective: to investigate the intake level of pickled vegetables and nitrite in pickles and pickles in Heilongjiang province, and to understand the intake of nitrite in pickled vegetables and pickles. The relationship between nitrite intake and oxidative stress in human body was further investigated. IL-17A- IFN- 纬 TNF- 伪 in plasma was detected by flow cytometry. IL-10, IL-6, IL-4, IL-2, and chemokine CXCL10. To explore the relationship between the levels of nitrite intake and human inflammatory factors in pickled vegetables and pickles. Methods: 80 people were selected from two villages of Hainan Township, Mudanjiang District, Heilongjiang Province. Samples of dietary pickles and pickles were collected in early December and the end of February for general demography. The contents of nitrite in pickled vegetables and pickles were determined by GB5009.33-2010. The intake of nitrite was calculated by combining the intake of pickled vegetables and pickles. The content of nitrite in plasma was determined by colorimetry. The content of SOD in plasma was determined by colorimetry. The levels of IL-17A- IFN- 纬 TNFN- 纬 TNFN- 10, IL-4, IL-4 and IL-2 in plasma of each group were detected by flow cytometry (CBA). Plasma CXCL10 levels were measured by ELISA double antibody sandwich method. The quartile spacing was grouped according to the quartile spacing of the intake, and the following quartile spacing was used as the reference group (control group). The levels of CAT in human plasma were detected and compared between the high intake group and the control group. The activity level of SOD was compared with that in the control group. The level of IL-17An- 纬 -TNFN- 纬 TNFN- 10 IL-10 IL-4 was compared with that in the control group. IL-2 and other cytokines and chemokine CXCL10 levels. Results: the general demographic characteristics of typical population in the investigated area were described. The first dietary intake of pickled vegetables was 195.98 卤79.32g / day per person. The dietary intake of pickled vegetables was 178.89 卤49.55g / day per person in the second survey. 70 samples of pickled vegetables were collected for the first time. 66 pickled vegetable samples were collected for the second time. The detection rate of nitrite was 70.6%. The average concentration of nitrite in plasma samples was 13.79 卤11.27 UM for the first time. The average nitrite concentration of 80 plasma samples collected in the second survey was 11.67 卤6.31 UM.The nitrite intake intervals were divided into groups. In the 1-4 group, the nitrite intake was:: 82.98 卤75.86ugn 273.77 卤33.81ugn 405.27 卤39.63ug respectively. 723.33 卤147.95ug.After the diet of pickled vegetables and pickles, the content of nitrite in human plasma was basically stable, and the activity of CAT in human plasma was observed after dietary pickled vegetables and pickles. The level of CXCL10 tended to stabilize and the activity of sod was inhibited to a certain extent. The plasma IL-17A IFN- 纬 TNFN- 10 IL-6 was found in the plasma of pickled vegetables and pickled vegetables. Conclusion: the intake of pickled vegetables and pickles in the investigated area was investigated. Dietary nitrite of pickled vegetable and pickled vegetable in typical population was within the limit of national standard. There was no difference in blood content of different pickled vegetable consumers. After ingesting nitrite in dietary pickled vegetable and pickled vegetable. SOD activity was inhibited to some extent. In this experiment, the changes of inflammatory factors in different pickled vegetable consumers were correlated with the intake of nitrite. With the increase of nitrite intake in pickled vegetables and pickled vegetables, the levels of cytokines such as IL-17, TNFN, IL-10, IL-6 and IL-4 in human plasma decreased.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R151.42

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