金纳米—汞复合物检测汞和金属硫蛋白的新方法研究

发布时间：2018-02-03 09:08

本文关键词： 汞金属硫蛋白金纳米粒子比色法四甲基联苯胺紫外分光光度法模拟酶　出处：《南华大学》2013年硕士论文　论文类型：学位论文

【摘要】：汞是一种严重影响人类健康的重金属，已被多个国家列为优先控制的环境污染物。汞现有的检测方法大多操作繁琐、仪器昂贵，因此，建立灵敏、简单、选择性高的新方法，对于环境中汞污染的监测和安全评价等均有重要意义。本文第二章建立了基于金纳米和四甲基联苯胺（TMB）催化比色检测汞离子的方法。在pH7.0的TAE缓冲液中，金纳米粒子与汞离子反应生成AuNPs-Hg过氧化物模拟酶，催化TMB显色，在9.14×10-8~7.69×10-6mol·L-1范围内，ΔA与汞离子浓度呈良好线性关系，回归方程为ΔA=0.001+0.091c(×10-6mol·L-1)，，相关系数为0.996，检出限为2.74×10-8mol·L-1。该方法简便、快速、稳定性好。本文第三章建立了基于金纳米-汞-TMB催化比色检测金属硫蛋白的新方法。在pH7.0的TAE缓冲液中，金属硫蛋白和金纳米粒子竞争结合汞离子，抑制AuNPs-Hg过氧化物模拟酶的生成，减弱TMB催化反应，在5.93×10-9~9.23×10-8mol·L-1范围内，ΔA与金属硫蛋白浓度呈良好线性关系，回归方程为ΔA=0.042+0.066c(×10-8mol·L-1)，相关系数为0.990，检出限为1.78×10-9mol·L-1。该方法简便、快速、灵敏、稳定性好。本文第四章建立了基于金纳米和金属硫蛋白紫外分光光度法检测汞离子的新方法。在pH7.0的TAE缓冲液中，汞离子导致金纳米-金属硫蛋白复合物聚集，使得紫外吸收发生改变，且在5.6×10-8mol·L-1~2.0×10-6mol·L-1范围内，体系(A626/A524)与汞离子的浓度有良好的线性关系，其回归方程为(A626/A524)=-0.0132+0.4072c(×10-6mol·L-1)，r=0.9961，检出限为1.68×10-8mol·L-1。该方法简单、快速、灵敏。
[Abstract]:Mercury is a serious human health effects of heavy metals, many countries have been listed as priority pollutants. Most of the existing detection method of mercury complex operation, expensive equipment, therefore, to establish a sensitive, simple and new method for high selectivity, the significance of mercury pollution in the environment monitoring and safety evaluation and so on.
The second chapter of this paper established gold nanoparticles and four methyl benzidine (TMB) method based on catalytic colorimetric detection of mercury ions. In the TAE pH7.0 buffer, gold nanoparticles and mercury ions generated AuNPs-Hg mimetic peroxidase, catalytic TMB color, 9.14 x 10-8~7.69 x 10-6mol - L-1 range, there is a good linear the relationship between A and the concentration of mercury, the regression equation was A=0.001+0.091c (10-6mol * L-1), the correlation coefficient was 0.996, the detection limit of 2.74 * 10-8mol L-1. the method is simple, rapid, good stability.
The third chapter of this paper established a new method for colorimetric detection of metallothionein - Mercury gold nanoparticles catalyzed by -TMB. Based on TAE buffer pH7.0, metallothionein and gold nanoparticles binding mercury ion, simulating AuNPs-Hg peroxide enzyme inhibition, decreased TMB catalytic reaction in 5.93 * 10-9~9.23 * 10-8mol - L-1 range A, which showed a good linear relationship with metallothionein concentration, the regression equation was A=0.042+0.066c (10-8mol * L-1), the correlation coefficient was 0.990, the detection limit of 1.78 * 10-9mol L-1. the method is simple, rapid, sensitive and good stability.
A new method was established in the fourth chapter, gold nanoparticles and metallothionein based on UV spectrophotometric detection of mercury ion. In the TAE pH7.0 buffer, mercury ions lead to gold nano - metal sulfur protein complex aggregation, makes the ultraviolet absorption change, and in the 5.6 * 10-8mol L-1~2.0 * 10-6mol L-1 within the scope of the system (A626/A524) has a good linear relationship with the concentration of mercury ion, the regression equation was (A626/A524) =-0.0132+0.4072c (10-6mol * L-1), r=0.9961, a detection limit of 1.68 * 10-8mol L-1. the method is simple, rapid and sensitive.

【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R114

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