原花青素对铁负荷大鼠肝脏功能及TfR2、hepcidin基因表达的影响

发布时间：2018-02-05 03:49

本文关键词： 铁负荷原花青素谷丙转氨酶谷草转氨酶 hepcidin TfR　出处：《中国农业大学学报》2017年08期 　论文类型：期刊论文

【摘要】：为探讨原花青素对铁负荷致大鼠肝损伤的影响,通过监测肝功能、病理切片及转铁蛋白受体-2(TfR2)、铁调素(hepcidin)基因表达,将40只SD大鼠随机分为对照组、原花青素组、铁负荷组和试验组(铁负荷+原花青素)。ICP法辅以肝脏普鲁士蓝染色判别铁负荷大鼠模型是否建立成功;相关试剂盒检测谷丙转氨酶(ALT)、谷草转氨酶(AST)的活性;HE染色观察肝组织形态学变化;Real-Time PCR检测肝组织中TfR2、hepcidin基因表达的变化。与对照组相比,铁负荷组Fe及ALT、AST水平显著升高(P0.05),肝组织结构严重紊乱,hepcidin基因的表达量显著下降(P0.05),TfR2基因的表达无明显差异;原花青素组Fe显著降低(P0.05),ALT、AST水平无显著性变化,肝组织结构正常,TfR2与hepcidin基因的表达量显著下降(P0.05)。与铁负荷组相比,试验组ALT、AST水平显著降低(P0.05),肝组织紊乱程度减轻,Fe含量、TfR2及hepcidin基因的表达量均略降低,但差异无统计学意义。原花青素可以抑制铁的摄入量;在机体铁过载状态下,原花青素抑制铁吸收的能力可以在一定程度上改善肝功能。
[Abstract]:In order to investigate the effect of procyanidins on liver injury induced by iron overload in rats, liver function, pathological sections and the expression of transferrin receptor -2TfR2, iron modulin hepcidinin were detected. Forty SD rats were randomly divided into control group and procyanidin group. Iron loading group and experimental group (iron loaded procyanidins. ICP method combined with liver Prussian blue staining to determine whether the iron load rat model was established successfully or not; The activity of alanine aminotransferase (alt) and glutamic oxaloacetic transaminase (AST) were detected by relevant kit. The changes of liver histomorphology were observed by HE staining. The expression of TfR2 hepcidin gene in liver tissue was detected by Real-Time PCR. Compared with the control group, Fe and ALT were detected in iron-loaded group. The level of AST increased significantly (P 0.05), and the expression of hepcidin gene decreased significantly (P 0.05). There was no significant difference in the expression of TfR2 gene. In proanthocyanidins group, Fe significantly decreased the level of alt AST in P0.05, and the liver tissue structure was normal. The expression of TfR2 and hepcidin gene decreased significantly (P 0.05). Compared with the iron load group, the alt AST level of the test group was significantly lower than that of the iron load group (P 0.05). The liver tissue disorder degree reduced the content of Fe and the expression of TfR2 and hepcidin genes, but there was no significant difference. Proanthocyanidins could inhibit the intake of iron. Under the condition of iron overload, the ability of proanthocyanidins to inhibit iron absorption can improve liver function to some extent.
【作者单位】：山西农业大学食品科学与工程学院;
【基金】：国家自然科学基金资助项目(31271826) 山西农业大学引进人才博士科研启动费(2013YJ30);山西农业大学科技创新基金(20132-02)
【分类号】：R151
【正文快照】： 铁是生物体必需的营养元素,在氧的运输与储存、电子传递、物质能量代谢、DNA、RNA及蛋白质的合成、血红素生物合成的调节等方面起着重要的生物学作用[1]。长期以来,人们由于过多的关注铁的营养价值及缺铁性贫血与防治,而忽略了铁过量潜在的危害,很容易引起机体铁负荷。此外也

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