不同分娩方式婴儿肠道菌群对比分析

发布时间：2018-02-05 03:59

本文关键词： 剖宫产术正常分娩婴儿肠道菌群　出处：《中国人民解放军医学院》2014年博士论文　论文类型：学位论文

【摘要】：目的：对比分析不同分娩方式出生婴儿肠道菌群差异方法：2012年4月至2012年11月在解放军总医院产科纳入不同分娩方式（顺产、剖宫产）的足月正常体重新生儿，收集胎粪及出生后1周、2周、1个月、2个月、3个月、6个月和1岁时粪便标本冻存于-80oC冰箱。跟踪随访婴儿喂养、生长发育、肠道情况以及用药情况（抗生素及益生菌制剂）。提取粪便细菌基因组DNA并扩增其16S rDNA的V4区，应用Illumina Miseq PE300平台测序，分析其肠道菌群结构。结果：1.最终随访得到喂养方式为母乳和配方奶粉混合喂养、且出生后1年内均未用过抗生素及益生菌制剂的婴儿共21例，其中剖宫产婴儿15例，顺产婴儿6例；2.胎粪中丰度最高的是变形菌门和硬壁菌门，其次是放线菌门和拟杆菌门，菌群组成以类杆菌属、双歧杆菌属、梭菌属、栖粪杆菌属、链球菌属和韦荣球菌属为主；3.婴儿出生后1年内，顺产组粪便样本总体菌群多样性略高于剖宫产组；顺产组粪便样本阿克曼菌属和瘤胃球菌属相对丰度高于剖宫产组；而剖宫产组粪便样本中海杆菌属和肠球菌属相对丰度高于顺产组；此外许多菌具有组间特异性，只出现于顺产组或只出现于剖宫产组；4.在顺产和剖宫产组各有几例婴儿其不同时间的粪便样品可以通过Unweighted unifrac聚类分别各自聚合成一类。结论：胎粪中细菌种类丰富，婴儿出生前肠道菌群已开始建立；顺产婴儿肠道菌群多样性略高于剖宫产婴儿，两组婴儿肠道菌群存在差异；婴儿出生后肠道菌群不同时间点比较存在一致性。
[Abstract]:Objective: to compare and analyze the differences of intestinal flora in infants with different delivery modes. Methods: from April 2012 to November 2012, normal full-term newborns with normal weight were enrolled in obstetrics department of PLA General Hospital. Meconium was collected and meconium was collected at 1 week and 2 weeks after birth. Stool samples were frozen in -80oC refrigerator for 1 month, 2 months, 3 months, 6 months and 1 year old. Intestinal tract and drug use (antibiotics and probiotics). Genomic DNA of fecal bacteria was extracted and the V4 region of 16s rDNA was amplified. The structure of intestinal flora was analyzed by Illumina Miseq PE300. Results 1. At the end of follow-up, there were 21 infants who were not treated with antibiotics and probiotics within 1 year after birth, including 15 cases of caesarean section. 6 cases were born naturally; 2. The highest abundance of meconium was Proteus and phylum durum, followed by Actinomycetes and Bacteroides. The flora consisted of Bacteroides, Bifidobacterium, Clostridium, and faeconium. Streptococcus and Veronella were dominant. 3. Within 1 year after birth, the fecal microflora diversity in the normal delivery group was slightly higher than that in the caesarean section group. The relative abundance of Acemonium and Rumen coccus in the faecal samples of the normal delivery group was higher than that in the caesarean section group. The relative abundance of the faecal samples of Cesarean section group and Enterococcus group was higher than that of normal delivery group. In addition, many bacteria have inter-group specificity, only in the normal delivery group or only in the cesarean section group; 4. In the normal delivery and caesarean section groups, there were several infants whose fecal samples at different times could be clustered into one group by Unweighted unifrac. There are plenty of bacteria in meconium. The establishment of intestinal flora before the birth of infants; The diversity of intestinal flora was slightly higher than that of caesarean section, and there was a difference between the two groups. There was consistency in intestinal flora at different time points after birth.
【学位授予单位】：中国人民解放军医学院
【学位级别】：博士
【学位授予年份】：2014
【分类号】：R174

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