钒暴露对大鼠小胶质细胞炎症反应和迁移的影响

发布时间：2018-02-05 04:31

本文关键词： 偏钒酸钠小胶质细胞炎性反应迁移　出处：《中国比较医学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的观察偏钒酸钠(Na VO3·2H2O)暴露对小胶质细胞炎症反应和迁移的影响,探讨钒神经毒性的相关机制。方法偏钒酸钠孵育原代培养的SD大鼠小胶质细胞,免疫荧光技术显示小胶质细胞形态的变化及其特异性标志物Iba1的表达,免疫印迹技术检测i NOS、COX-2、ERK和p-ERK蛋白表达,酶联免疫吸附实验检测炎症因子TNF-α,IL-1β的释放水平;构建划痕迁移模型,免疫荧光技术记录偏钒酸钠对小胶质细胞迁移的影响。结果偏钒酸钠孵育小胶质细胞后,神经小胶质细胞形态由静息态的分支状向吞噬细胞样形态转变,其特异性标志物Iba1表达显著增加;i NOS和COX-2的蛋白表达和TNF-α,IL-1β释放水平与对照组相比较均显著升高;偏钒酸钠促进小胶质细胞的迁移。结论偏钒酸钠显著促进了小胶质细胞的炎症反应和迁移。
[Abstract]:Objective to observe the effect of sodium metavanadate (Na VO3 路2H 2O) exposure on inflammatory response and migration of microglia. Methods the primary cultured microglia of SD rats were incubated with sodium metavanadate. The morphological changes of microglia and the expression of specific marker Iba1 were detected by immunofluorescence technique. The expression of iNOS COX-2 ERK and p-ERK protein was detected by Western blot, and the release level of inflammatory factor TNF- 伪 and IL-1 尾 was detected by enzyme-linked immunosorbent assay (Elisa). The effect of sodium metavanadate on the migration of microglia was recorded by immunofluorescence technique. Results the microglia were incubated with sodium metavanadate. The morphology of neural microglia changed from resting branching to phagocyte-like, and the expression of specific marker Iba1 increased significantly. The protein expression of I NOS and COX-2 and the level of IL-1 尾 release of TNF- 伪 were significantly higher than those of the control group. Conclusion Sodium metavanadate can promote the inflammation and migration of microglia.
【作者单位】：湖南省职业病防治院;
【基金】：湖南省卫生与计划生育委员会科研基金(B2015-318)
【分类号】：R114
【正文快照】： yuxw0714@163.com钒是地球上广泛分布的微量元素。钒的大量开发与应用引发的环境问题日益严重,生产过程中产生的废气成为大气中钒的重要来源[1,2],厂区及附近的土壤、水源大都受到污染,尤以钒、锰、铜、铅为主[3,4],存在严重职业危害与环境污染。国内外研究显示,钒具有神经毒

【相似文献】