镉致HEK293细胞氧化损伤及细胞凋亡

发布时间：2018-02-05 21:52

本文关键词： 镉 HEK 活性氧丙二醛谷胱甘肽过氧化物酶超氧化物歧化酶细胞凋亡　出处：《环境与职业医学》2017年02期 　论文类型：期刊论文

【摘要】：[目的]探讨镉致人胚胎肾HEK293细胞氧化损伤和凋亡的情况。[方法]对HEK293细胞使用0、30、60、120μmol/L浓度的氯化镉(CdCl_2)染毒1、3、6、12 h后,MTT法测定细胞生长情况,流式细胞术检测细胞中活性氧(ROS)含量和细胞凋亡,用硫代巴比妥酸法检测细胞内丙二醛(MDA)含量,用比色法测定谷胱甘肽过氧化物酶(GSH-PX)活力,用水溶性四唑盐法检测细胞中超氧化物歧化酶(SOD)活力。[结果]不同浓度的CdCl_2作用不同时间,细胞贴壁性减弱,细胞变圆,伪足消失。随着CdCl_2浓度的增高,作用时间延长,细胞的生长受到抑制,在CdCl_2浓度为120μmol/L,染毒时间为12 h时达到峰值。随着作用时间的延长,30μmol/L和60μmol/L CdCl_2染毒组细胞凋亡率逐渐增加(P趋势=0.001,P趋势=0.009);当作用时间分别为1、3、6 h,随着CdCl_2浓度的增加,凋亡率逐渐增加(P趋势=0.003或P趋势=0.001)。相同染毒时间,细胞内MDA、ROS含量随着CdCl_2浓度的升高而增高(P趋势0.001或P趋势=0.001);当CdCl_2浓度分别为30、60、120μmol/L时,随着染毒作用时间的延长,MDA和ROS含量逐渐增高(均P趋势0.05)。与0μmol/L CdCl_2组比较,30、60、120μmol/L组细胞中SOD和GSH-PX活力均降低(P0.05),且随着CdCl_2浓度的增加细胞中SOD和GSH-PX活力呈下降趋势(P趋势0.001);当染毒浓度分别为30、60、120μmol/L时,细胞SOD和GSH-PX活力随着作用时间的延长而降低(均P趋势0.01)。[结论]镉可导致HEK293细胞氧化损伤和细胞凋亡。
[Abstract]:[Objective] to investigate the oxidative damage and apoptosis of human embryonic kidney HEK293 cells induced by cadmium. [Methods] HEK293 cells were treated with cadmium chloride (CDCL _ 2) at a concentration of 60 ~ 120 渭 mol/L for 12 h. MTT method was used to detect cell growth, flow cytometry was used to detect the content of reactive oxygen species (Ros) and apoptosis, and thiobarbituric acid method was used to detect the content of malondialdehyde (MDA). The activity of glutathione peroxidase (GSH-PX) and the activity of superoxide dismutase (SOD) in cells were determined by colorimetric method and water-soluble tetrazolium salt method. [Results: at different concentrations of CdCl_2, the adhesion of cells decreased, the cells became round and pseudopodia disappeared. With the increase of CdCl_2 concentration and the prolongation of the time of action, the growth of cells was inhibited. When the concentration of CdCl_2 was 120 渭 mol / L, the exposure time reached the peak at 12 h. The apoptosis rate of 30 渭 mol/L and 60 渭 mol/L CdCl_2 groups increased gradually. With the increase of CdCl_2 concentration, the apoptosis rate increased gradually when the action time was 1 ~ 3 ~ 6 h, respectively. The P trend was 0.003 or P trend was 0.001g. The same exposure time was observed. The content of MDA-Ros in cells increased with the increase of CdCl_2 concentration. When the concentration of CdCl_2 was 30 ~ 60,120 渭 mol/L, the time of exposure was prolonged. The contents of MDA and ROS increased gradually (P < 0.05). Compared with 0 渭 mol/L CdCl_2 group, the content of MDA and ROS increased gradually (P < 0.05). The activities of SOD and GSH-PX in the cells of 120 渭 mol/L group were decreased by P0.05). The activity of SOD and GSH-PX decreased with the increase of CdCl_2 concentration. The activities of SOD and GSH-PX decreased with the prolongation of the exposure time (P < 0.01) when the exposure concentration was 30 ~ 60,120 渭 mol/L, respectively. [Conclusion cadmium can induce oxidative damage and apoptosis of HEK293 cells.
【作者单位】：山西医科大学公共卫生学院流行病学教研室;
【基金】：国家自然科学基金(编号:81273040) 山西省回国留学人员科研资助项目(编号:2015-055)
【分类号】：R114

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