气管插管法建立矽肺大鼠模型方法的改进

发布时间：2018-02-06 00:04

本文关键词： 气管插管矽肺肺纤维化大鼠模型　出处：《现代预防医学》2017年22期 　论文类型：期刊论文

【摘要】：目的介绍一种经济实用、成功率高的气管插管方法来制备矽肺大鼠模型。方法将20只SPF级Wistar雄性大鼠随机分为对照组和矽肺模型组,2组均使用自制实验器具通过直视下行插管法给药,经检验插管成功后向对照组气管注入1 ml生理盐水,模型组气管注入1 ml Si O2悬浊液。染毒后28 d取出肺组织行HE和Masson染色,应用碱水解法检测肺组织中羟脯氨酸含量。结果 2组大鼠造模过程中无死亡;造模后对照组大鼠生长状况良好,模型组大鼠生长缓慢并伴有毛枯、喘息等症状;肺组织病理学检查发现,对照组大鼠肺组织结构正常,无明显纤维化;模型组大鼠肺组织出现典型的肺泡炎和纤维化改变。模型组大鼠羟脯氨酸含量明显高于对照组,差异有统计学意义(P0.01)。结论组织病理学检查及羟脯氨酸测定结果提示经改良的气管插管注药方法成功复制了矽肺模型,操作简便,死亡率低,可加以推广。
[Abstract]:Objective to introduce a practical and economical method of endotracheal intubation to establish silicosis rat model. Methods Twenty SPF grade Wistar male rats were randomly divided into two groups: control group and silicosis model group. The two groups were administered with self-made experimental instruments through direct vision intubation. After successful intubation, 1 ml of normal saline was injected into the trachea of the control group. In the model group, 1ml Sio _ 2 suspension was injected into the trachea. Lung tissues were taken out for HE and Masson staining 28 days after exposure. The content of hydroxyproline in lung tissue was detected by alkaline hydrolysation. The growth status of the control group was good after modeling, and the growth of the model group was slow and accompanied with symptoms such as hair blight and wheezing. Lung histopathological examination showed that the control group had normal lung tissue structure and no obvious fibrosis. There were typical changes of alveolitis and fibrosis in the lung tissue of the model group, and the content of hydroxyproline in the model group was significantly higher than that in the control group. Conclusion the results of histopathological examination and hydroxyproline determination suggest that the modified endotracheal intubation method successfully duplicated the silicosis model with simple operation and low mortality. It can be popularized.
【作者单位】：济南大学山东省医学科学院医学与生命科学学院山东省职业卫生与职业病防治研究院;山东省医学科学院山东省职业卫生与职业病防治研究院;山东大学附属省立医院中毒与职业病科;
【基金】：山东省医学科学院医药卫生科技创新工程山东省重点研发计划(编号:2016GSF201047) 山东省自然科学基金(编号:ZR2015YL046)
【分类号】：R135.2;R-332

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