褪黑素对丙烯酰胺致睾丸毒性保护作用的机制（英文）

发布时间：2018-02-10 19:56

本文关键词： 褪黑素丙烯酰胺睾丸毒性凋亡氧化损伤　出处：《中山大学学报(医学科学版)》2017年04期 　论文类型：期刊论文

【摘要】：【目的】探讨褪黑素(MT)对丙烯酰胺(AA)诱发睾丸毒性的改善作用及机制。【方法】动物随机分为3组:对照组,AA染毒组和AA+MT治疗组,每组各12只大鼠。AA染毒的动物连续4周每日灌胃AA(15 mg/kg)。从染毒3周后开始,治疗组动物先每日腹腔注射MT 10 mg/kg(连续1周),30 min后再进行AA染毒。对照组动物仅注射同等剂量的生理盐水。随后分别进行线粒体膜电位、TUNEL、免疫印迹和电镜检测。【结果】研究结果表明AA可使睾丸组织线粒体膜电位下降(P0.05),且MT可令其恢复(P0.05)。和对照组相比,MT可下调睾丸组织中Bax的表达;上调Bcl-2的表达。TUNEL结果进一步证实MT可减缓解睾丸组织中细胞凋亡现象。免疫印迹结果表明AA染毒组Bcl-2/Bax和Bcl-xL/Bak的比值较对照组降低(P皆0.05),而MT治疗组中它们的比值升高(P皆0.05)。AA染毒组中Cyt-c,Casp-3,p53和NF-κB的表达水平较对照组明显升高(P皆0.05),较MT治疗组明显下降(P皆0.05)。电镜结果证实MT可减轻AA造成的睾丸曲细精管中线粒体结构受损。【结论】MT可能通过和线粒体通路相关的抗凋亡功能,从而缓解AA造成的睾丸毒性。
[Abstract]:[objective] to investigate the effect and mechanism of melatonin (MTT) on testicular toxicity induced by acrylamide (AA). [methods] Animals were randomly divided into three groups: control group treated with AA and AA MT treated group. 12 rats in each group were given AA(15 mg / kg of AA(15 daily for 4 weeks. In the treatment group, MT 10 mg / kg was injected intraperitoneally for 30 min and then AA was administered to the control group. The control group was treated with the same dose of normal saline. Then the mitochondrial membrane potential was detected by Tunel, immunoblotting and electron microscopy. [. Results the results showed that AA could decrease the mitochondrial membrane potential of testis and restore the expression of Bax in testis. The up-regulated expression of Bcl-2. Tunel results further confirmed that MT can reduce apoptosis in testis tissue. Western blot showed that the ratio of Bcl-2/Bax and Bcl-xL/Bak in AA exposed group was lower than that in control group (0.05%), while in MT treatment group, the ratio increased. The expression levels of Cyt-casp-3P and NF- 魏 B in both groups were significantly higher than those in the control group, and decreased significantly compared with those in the MT treatment group. The results of electron microscope showed that MT could reduce the mitochondrial structure damage in the testicular seminiferous duct caused by AA. [conclusion] the results of electron microscope showed that MT could reduce the damage of mitochondria structure in the testicular seminiferous duct caused by AA. MT may pass through anti-apoptotic functions associated with mitochondrial pathways, This alleviates the testicular toxicity caused by AA.
【作者单位】：广东药科大学基础学院人体解剖学教研室;广东药科大学基础学院生理学教研室;
【基金】：广东省自然科学基金(2014A030310455) 广州市珠江科技新星专项(201710010002)
【分类号】：R114

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