雾霾和沙尘暴天气细颗粒物对人角质细胞促炎症作用和过敏反应的影响

发布时间：2018-02-24 04:37

本文关键词： 细颗粒物人角质细胞促炎症作用过敏反应　出处：《毒理学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的观察细颗粒物PM_(2.5)对人角质细胞HaCaT的促炎症作用和过敏反应的影响。方法在雾霾天气和沙尘暴天气下分别收集PM_(2.5),用多次超声水浴法提取PM_(2.5)全颗粒,雾霾天气和沙尘暴天气下收集制备的PM_(2.5)分别记为PM_(2.5)non-dust即PM_(2.5)(N)、PM_(2.5)dust即PM_(2.5)(D)。实验组分为PM_(2.5)(N)、PM_(2.5)(D)及PM_(2.5)+2,4-二硝基氯苯(2,4-dinitrochlorobenzol,DNCB)联合作用组,采用MTT实验、实时荧光定量PCR实验、ELISA检测测定各处理组作用后细胞存活率、相关促炎症因子IL-18、IL-1α、TNF-α表达变化。结果与对照组相比HaCaT细胞经各组处理后的细胞存活率差异无统计学意义(P0.05)。HaCaT细胞经PM_(2.5)(N)处理后,IL-1α、TNFα的胞内mRNA表达差异无统计学意义(P0.05);而经PM_(2.5)(D)处理后IL-1α、TNFα的表达明显增加,变化倍数分别上调为2.65倍(P0.01)、1.69倍(P0.05);HaCaT细胞经PM_(2.5)(N)、PM_(2.5)(D)处理后IL-18的胞外释放量与对照相比均有显著增加(P0.01),而胞内表达水平存在差异;且PM_(2.5)预处理可明显拮抗化学致敏剂DNCB导致的IL-18细胞外蛋白含量增加(P0.05)。颗粒物组分分析显示,TNF-α的产生与PAHs(r=0.929)、WSOC(r=0.905)成正相关;而IL-18和IL-1α的产生与PAHs(r=-0.901,r=-0.864)、WSOC(r=-0.847,r=-0.919)成负相关。结论本研究表明了不同组分PM_(2.5)引发皮肤角质细胞免疫反应的异同;沙尘天气比雾霾天气的PM_(2.5)可能具有更强的促炎症效应;而雾霾天气的PM_(2.5)的致敏反应可能更为持久,颗粒物的致敏反应可能通过TLR2/4等促免疫通路导致。
[Abstract]:Objective to observe the effects of fine particulate matter (PMSP 2.5) on the inflammatory and allergic reaction of human keratinocytes (HaCaT). Methods under the weather of haze and sandstorm, PMS2.5 was collected and extracted by ultrasonic water bath. Haze and dust storms were recorded as PM_(2.5)non-dust. The experimental group was divided into two groups: PMV2.5D and PMS-2.5-D). The experimental group was divided into two groups: 24-dinitrochlorobenzolium (DNCBB) and 24-dinitrochlorobenzolium (DNCBB), and the experimental group was divided into two groups, namely, PM2. 5 (DNCBs) and PM20 (2. 4 dinitrochlorobenzolium DNCBs). The experimental group was divided into two groups: PM2. 5 (DNCBs) and PM20 (24-dinitrochlorobenzolium) (DNCBs). Real-time fluorescence quantitative PCR assay and Elisa were used to detect the survival rate of the treated cells. Results compared with the control group, there was no significant difference in the cell survival rate of HaCaT cells treated with P0.05N. HaCaT cells were treated with P0.05N. There was no significant difference in the intracellular mRNA expression of IL-1 伪 -TNF- 伪 after treated with P0.05. HaCaT cells were compared with the control group (P0.05), and there was no significant difference in the expression of mRNA between the two groups after the treatment of IL-18 and IL-1 伪, compared with the control group, there was no significant difference in the intracellular mRNA expression between the two groups. The expression of IL-1 伪 -TNF- 伪 was significantly increased after the treatment of PMC 2.5, and the expression of TNF- 伪 was significantly increased after treatment. The changes were increased to 2.65 times (P 0.01) and 1.69 times (P 0.05). The extracellular release of IL-18 was significantly higher than that of the control, and the intracellular expression level was different between the two groups. In addition, P0. 05) pretreatment could significantly antagonize the increase of extracellular protein content of IL-18 induced by chemical sensitizer DNCB. The analysis of particulate composition showed that there was a positive correlation between the production of TNF- 伪 and the production of PAHs / TNF- 伪 (0. 905). However, the production of IL-18 and IL-1 伪 was negatively correlated with the expression of IL-18 and IL-1 伪 in the skin keratinocytes. Conclusion the results show that the IL-18 and IL-1 伪 may have a stronger inflammatory effect on the skin keratinocytes than those on the haze weather, and that the different components of PMS-2.5) may have a stronger pro-inflammatory effect than that of the PMS-0.901r-0.864. Conclusion the present study shows that the different components of PMS-2.5) induce the keratinocyte immune response of the skin, and that the sand dust weather may have a stronger pro-inflammatory effect than that of the haze weather. However, the sensitizing reaction of haze may be more persistent, and the sensitization of particulate matter may be caused by TLR2/4 and other immune pathways.
【作者单位】：北京大学公共卫生学院;南京大学模式动物研究所;
【基金】：国家自然科学基金(21577004) 北京大学青年人才基金(BMU20130340) 北京市自然科学基金(7162104)
【分类号】：R122

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