虾青素对高脂诱导小鼠非酒精性脂肪肝的保护作用

发布时间：2018-02-24 23:00

本文关键词： 虾青素高脂饮食非酒精性脂肪性肝病炎症因子　出处：《营养学报》2016年04期 　论文类型：期刊论文

【摘要】：目的通过高脂饮食诱导的小鼠非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)模型研究虾青素对NAFLD的保护作用。方法将30只雄性C57BL/6J小鼠随机分为对照组、高脂组和干预组,每组10只。对照组饲喂低脂饲料,高脂组饲喂高脂饲料,干预组饲喂虾青素饲料(每kg高脂饲料添加2g虾青素)。16w后,称量各组小鼠体质量、肝质量并计算肝脏指数;测定小鼠肝功能、血脂及炎症因子水平;肝组织苏木素和伊红(hematoxylin and eosin,HE)染色观察组织形态学变化。结果高脂组小鼠肝脏指数与对照组相比显著降低(P0.05),补充虾青素后与高脂组相比显著升高(P0.05)。高脂组小鼠血清谷草转氨酶(alanine aminotransferase,ALT)、谷丙转氨酶(aspartate aminotransferase,AST)、甘油三酯(triglyceride,TG)、总胆固醇(cholesterol,TC)、低密度脂蛋白(low-density lipoprotein,LDL)水平与对照组相比显著升高(P0.05);干预组小鼠血清ALT、AST、TG、TC水平与高脂组相比显著降低(P0.05),高密度脂蛋白(high-density lipoprotein,HDL)水平显著升高(P0.05)。高脂组小鼠肝脏TG水平与对照组相比显著升高(P0.05),补充虾青素后与高脂组相比显著降低(P0.05),各组肝脏TC水平无明显差异。HE染色结果表明,高脂组肝组织可见大量脂肪变性,而干预组脂肪变性程度明显减轻。另外,高脂组小鼠血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-6(interleukin-6,IL-6)及C反应蛋白(C-reactive protein,CRP)浓度与对照组相比显著升高(P0.05),补充虾青素后分别降低了24.03%、18.45%、27.16%。结论虾青素可通过改善肝功能、血脂水平及抑制炎症因子释放等途径减缓高脂饮食诱导的NAFLD。
[Abstract]:Objective to study the protective effect of astaxanthin on NAFLD in mice with non-alcoholic fatty liver disease induced by high-fat diet. Methods Thirty male C57BL / 6J mice were randomly divided into control group, hyperlipidemia group and intervention group. The control group was fed with low-fat diet, the high-fat group was fed with high-fat diet, the intervention group was fed with astaxanthin diet (2g astaxanthin per kg high-fat diet) for 16 weeks, the body weight, liver quality and liver index of each group were measured. Liver function, blood lipid and inflammatory factors were measured. Liver tissue hematoxylin and eosin (HEH) staining was used to observe the histomorphologic changes. Results the liver index of the hyperlipidemic group was significantly lower than that of the control group, and that of astaxanthin supplementation was significantly higher than that of the hyperlipidemia group. The serum of hyperlipidemia group was significantly higher than that of the hyperlipidemia group. The levels of alanine aminotransferase, alanine aminotransferase, alanine aminotransferase, aspartate aminotransferase, triglyceride-triglyceride, total cholesterol cholesterol, low-density lipoprotein and low density lipoprotein (LDLL) in the intervention group were significantly higher than those in the control group (P 0.05), and the serum ALT AST TGT level in the intervention group was significantly lower than that in the hyperlipidemia group (P 0.05), and high density lipoprotein (LDL) level in the intervention group was significantly lower than that in the hyperlipidemia group (P 0.05). Compared with the control group, the liver TG level of the high-fat group was significantly higher than that of the control group, and that of astaxanthin supplementation group was significantly lower than that of the high-fat group. There was no significant difference in liver TC level between the two groups. The results of HE staining showed that there was no significant difference in liver TC level between the high fat group and the control group. A large number of fatty degeneration was observed in liver tissue of high-fat group, while the degree of steatosis was significantly reduced in intervention group. The serum levels of tumor necrosis factor- 伪 (TNF- 伪), interleukin-6 (IL-6) and C-reactive protein (CRP6) of mice in hyperlipidemia group were significantly higher than those in control group, and the levels of astaxanthin decreased 18.4527.165.Conclusion astaxanthin can improve liver function. The levels of blood lipids and inhibiting the release of inflammatory factors inhibited NAFLD induced by high fat diet.
【作者单位】：广东医学院公共卫生学院;中山大学公共卫生学院;
【基金】：国家自然科学基金(No.81302416) 广东省科技计划项目(No.2013B031800016;No.2014A020212297) 广东省高校优秀青年教师培养计划项目(No.YQ201405) 东莞市科技计划项目(No.2014108101053)
【分类号】：R151

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