萘乙酸对小鼠肝细胞增殖与凋亡的影响

发布时间：2018-02-25 10:11

本文关键词： 萘乙酸肝细胞细胞增殖细胞凋亡　出处：《毒理学杂志》2015年03期 　论文类型：期刊论文

【摘要】：目的探讨萘乙酸对小鼠肝细胞增殖与凋亡的影响。方法将50只健康昆明种小鼠随机分为5个组,即正常对照组、高剂量组、中剂量组、低剂量组和阳性对照组,每组10只,雌雄各半,试验周期为4个周。用MTT法检测肝细胞增殖活性,用免疫组化的方法检测肝组织的PCNA、Bcl-2和Caspase-3表达水平。结果高剂量组和正常对照组的肝细胞增殖活性分别为0.794±0.019和1.055±0.019,差异有统计学意义(P0.05)。高剂量NAA组PCNA阳性表达率为11.2%,正常对照组为33.1%,经检验差异有统计学意义(P0.05)。高、中剂量NAA组Caspase-3阳性表达率分别为37.9%和8.2%,正常对照组为6.3%,经检验差异有统计学意义(P0.05)。高剂量组和正常对照组Bcl-2阳性表达率分别为12.1%和31.9%,差异有统计学意义(P0.05)。结论萘乙酸能够抑制小鼠肝细胞增殖活性和PCNA、Bcl-2表达水平,并促进Caspase-3的表达,促使凋亡的发生。
[Abstract]:Objective to investigate the effects of naphthalene acetic acid on the proliferation and apoptosis of hepatocytes in mice. Methods Fifty healthy Kunming mice were randomly divided into 5 groups: normal control group, high dose group, middle dose group, low dose group and positive control group. The experimental period was 4 weeks. The proliferative activity of hepatocytes was detected by MTT assay. Results the proliferative activity of hepatocytes in high dose group and normal control group was 0.794 卤0.019 and 1.055 卤0.019 respectively, the difference was statistically significant (P 0.05). The positive rate of PCNA expression in high dose NAA group was 11.2%. The normal control group was 33. 1%. The difference was statistically significant (P 0. 05). The positive expression rates of Caspase-3 were 37.9% and 8.2 in the middle dose NAA group and 6.3in the normal control group, respectively. The difference was statistically significant (P 0.05). The positive rate of Bcl-2 expression in the high dose group and the normal control group was 12.1% and 31.9, respectively. Conclusion Naphthalene has statistical significance. Acetic acid inhibited the proliferation activity and the expression of PCNA- Bcl-2 in mouse hepatocytes. And promote the expression of Caspase-3, promote the occurrence of apoptosis.
【作者单位】：青岛市疾病预防控制中心;青岛大学医学院;
【分类号】：R114

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