绿茶提取物与维生素C和番茄红素的协同抗氧化作用

发布时间：2018-02-28 08:09

本文关键词： 绿茶提取物抗氧化活性丙二醛超氧化物歧化酶谷胱甘肽过氧化物酶还原型谷胱甘肽蛋白质羰基　出处：《卫生研究》2017年04期 　论文类型：期刊论文

【摘要】：目的研究绿茶提取物联合维生素C和番茄红素对D-半乳糖致氧化损伤模型小鼠体内抗氧化活性的影响。方法 72只小鼠按体重随机抽取12只作为空白对照组,其余用D-半乳糖1.0 g/kg腹腔注射造模,6周后按照丙二醛(MDA)水平随机分为:模型对照组、绿茶提取物组和低、中、高3个剂量绿茶提取物+维生素C+番茄红素混合受试物组。模型对照组蒸馏水灌胃,绿茶提取物组0.87 g/kg单一绿茶提取物灌胃,低、中、高剂量混合受试物组分别按0.15、0.29、0.87 g/kg混合物灌胃。4周后用分光光度法测血清与肝脏内MDA含量、超氧化物歧化酶(SOD)活性、还原型谷胱甘肽(GSH)含量、谷胱甘肽过氧化物酶(GSH-Px)活性、蛋白质羰基含量。结果与氧化损伤模型对照组相比,高剂量混合物组、绿茶提取物组均能增高小鼠血清和肝组织内GSH-Px、SOD活性,降低蛋白质羰基、MDA含量(P0.01或P0.05);与绿茶提取物组比较,高剂量混合物组GSH-Px、SOD活性增高,MDA、蛋白质羰基含量降低(P0.01或P0.05)。结论绿茶提取物联合维生素C和番茄红素比单一使用绿茶提取物抗氧化效果更好,三者表现出良好的协同抗氧化能力。
[Abstract]:Objective to study the effect of green tea extract combined with vitamin C and lycopene on antioxidant activity in mice with oxidative injury induced by D-galactose. The rest were randomly divided into three groups: model control group, green tea extract group and low, middle, low, low, middle, low, low, low, low, low, low, low, low, low, low, low, low, low, low, low, low, low, low, low, low, low, High dose green tea extract vitamin C lycopene mixed test group. Model control group distilled water gastric perfusion, green tea extract group 0.87 g / kg single green tea extract gavage, low, medium, low. The serum and liver MDA content, superoxide dismutase (SOD) activity, reduced glutathione glutathione (GSH) content and glutathione peroxidase (GSH-Px) activity in serum and liver were measured by spectrophotometry after oral administration of 0.15U 0.29g / kg 0.87 g / kg mixture. Results compared with the oxidative injury model control group, the high dose mixture group and green tea extract group could increase the SOD activity in serum and liver tissue, and decrease the MDA content of protein carbonyl group (P0.01 or P0.05), compared with the green tea extract group. In high dose mixture group, SOD activity increased and protein carbonyl group decreased P0.01 or P0.05.Conclusion Green tea extract combined with vitamin C and lycopene has better antioxidant effect than green tea extract alone.
【作者单位】：哈尔滨医科大学公共卫生学院营养与食品卫生系;
【基金】：国家自然科学基金(No.81273062)
【分类号】：R151

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