核桃内种皮提取物对小鼠脑损伤的改善作用

发布时间：2018-03-01 19:38

  本文关键词： 核桃内种皮 多酚 衰老 脑 氧化损伤　出处：《食品研究与开发》2017年11期 　论文类型：期刊论文

【摘要】：研究核桃内种皮多酚提取物对D-半乳糖致衰小鼠脑组织氧化损伤程度的调节。选取ICR雄性小鼠60只,随机分为5组,每组12只,对模型对照组和剂量组小鼠采取颈部皮下注射D-半乳糖[120 mg/(kg·bw·d)]制造衰老模型,低、中、高剂量组分别灌胃200、400、800 mg/(kg·bw·d)的核桃内种皮多酚提取物,模型对照组灌胃等量的双蒸水。给药7周后,通过旷场试验、紧绳实验和平衡木实验检测每组小鼠间的行为学差异。8周后,取脑组织,测定脑组织匀浆中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活力和总抗氧化能力(T-AOC)及丙二醛(MDA)的含量。结果表明,与模型对照组相比,核桃内种皮多酚提取物可显著提高小鼠的运动和探索能力,增强小鼠的兴奋性和对环境的适应性。同时,脑组织中SOD、GSH-Px活力和T-AOC含量较模型组有显著升高。这表明核桃内种皮多酚提取物可减轻D-半乳糖导致的行为迟钝,并能减轻小鼠脑部的氧化损伤。
[Abstract]:To study the effect of polyphenol extract from inner seed coat of walnut on oxidative damage of brain tissue in aging mice induced by D-galactose, 60 male ICR mice were randomly divided into 5 groups, 12 rats in each group. The aging model was induced by subcutaneous injection of D-galactose (120 mg/(kg 路BW 路d) into the neck of mice in the model control group and the dose group. The low, middle and high dose groups were given 200,400,800 mg/(kg 路BW 路d of polyphenol extract from the inner coat of walnut respectively. The model control group was given the same amount of double-steamed water. After 7 weeks of administration, the behavioral differences between each group were detected by open-field test, tight-rope test and balance beam test. The activities of superoxide dismutase (SOD), catalase (cat), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) and malondialdehyde (MDA) in brain homogenate were determined. The polyphenol extract from the inner seed coat of walnut can significantly improve the exercise and exploration ability of mice, enhance the excitability and adaptability to the environment of the mice, at the same time, The activity of GSH-Px and the content of T-AOC in brain tissue were significantly higher than those in the model group, which indicated that the polyphenol extract from walnut seed coat could alleviate the behavioral retardation induced by D-galactose and reduce the oxidative damage to the brain of mice.
【作者单位】： 食品营养与安全省部共建教育部重点实验室食品工程与生物技术学院天津科技大学;天津科技大学新农村发展研究院;
【基金】：天津科技大学新农村发展研究院开放基金资助项目(XNC201511) 教育部科技发展中心“蓝火计划”产学研联合创新项目衡水专项2014年度项目(2014-LHJH-HSZX-017)
【分类号】：R151
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本文编号：1553318