多氯联苯对男性生殖影响的研究

发布时间：2018-03-02 08:33

  本文关键词： 生殖健康 多氯联苯 快速溶剂萃取 气相色谱质谱　出处：《华南理工大学》2013年硕士论文　论文类型：学位论文

【摘要】：随着工业生产的发展，环境污染引起的健康问题逐年加剧，其中对男性生殖健康的影响尤为突出。多氯联苯是一类广泛存在的环境有机污染物，具有不易降解、环境持久性、生物积聚性，对人体生殖健康存在潜在危害性。 本研究用快速溶剂萃取（ASE）法和气相色谱质谱（GC-MS）法测定血液及精液样品中的6种指示性多氯联苯（PCB52、PCB101、PCB118、PCB138、PCB153、PCB180）的浓度。旨在探讨全血和精液中PCBs污染水平与男性生殖障碍之间的关系，建立简便快捷的分析方法，为相关生殖健康研究提供数据依据，也可为我国的PCBs污染控制提供背景依据。 本研究建立了人体全血和精液ASE前处理方法及GC-MS的检测PCBs的方法，实验结果PCBs加标回收率为：53-83%；各PCBs单体的方法检出限为0.24-1.15ng/g。本方法符合分析全血和精液此类样本的需要。 本研究通过不育门诊就诊的患者采集血液和精液，分析样本中PCBs的浓度。发现血液中PCB52平均浓度为4.47ng/g，PCB101为2.70ng/g，PCB118未检出，PCB138为8.14ng/g，PCB153为1.83ng/g，PCB180为10.24ng/g。精液样本中PCB52浓度为3.84ng/g，PCB101为2.52ng/g，PCB118为0.45ng/g，PCB-138为4.42ng/g，PCB153为6.04ng/g，PCB180为6.86ng/g。结果表明，不育患者的血液和精液中的PCBs浓度远高于正常人平均水平。 通过不育患者与正常人血液及精液中PCBs浓度的对比发现，，不育患者血液及精液中的PCBs浓度较高。其中影响PCBs浓度的因素有：职业、居住地、BMI、年龄等。从事个体经营的人血液及精液中的PCBs浓度较高，而IT行业的浓度较低；居住在市区的患者的精液中PCBs浓度比居住在郊区的患者PCBs浓度高，而血液则相反；BMI适中的人体内PCBs浓度较低，过轻或过重都将导致PCBs浓度较高；研究还发现，实验还发现患者的年龄越大血液和精液中总PCBs浓度越低。 目前，国内外对PCBs与男性生殖健康的关系的研究还处于探索阶段。本实验为该方面的流行病学研究及环境激素所引起的生殖健康问题提供数据支持。
[Abstract]:With the development of industrial production, the health problems caused by environmental pollution are aggravated year by year, especially on male reproductive health. Polychlorinated biphenyls (PCBs) are a class of environmental organic pollutants, which are difficult to degrade and persistent in the environment. Biological accumulation has potential harm to human reproductive health. In this study, the rapid solvent extraction (ASE) method and gas chromatography-mass spectrometry (GC-MS) were used to determine the concentration of six indicative PCB52PCB101C PCB101P PCB118PIB138PIB153PCB180 in blood and semen samples. The aim of this study was to investigate the relationship between the levels of PCBs contamination in whole blood and semen and male reproductive disorders. To establish a simple and rapid analysis method, to provide data basis for related reproductive health research, but also to provide background basis for PCBs pollution control in China. In this study, the pretreatment method of ASE in human whole blood and semen and the method for detecting PCBs in human whole blood and semen were established. The results showed that the recovery rate of PCBs plus standard was 53-83.The detection limit of each PCBs monomer was 0.24-1.15ng / g. This method was suitable for the analysis of whole blood and semen samples. In this study, blood and semen were collected from infertile outpatients. Analysis of the concentration of PCBs in the sample. It was found that the average concentration of PCB52 in the blood was 4.47 ng / g / g PCB101 = 2.70 ng / g / g PCB118. No PCB138 was found to be 8.14 ng / g / g PCB153 = 1.83 ng / g / g / g PCB180 = 10.24 ng / g / g. The PCB52 concentration in semen samples was 3.84 ng / g / g / g PCB101 and 2.52 ng / g / g PCB118 was 0.45 ng / g / 138, 4.42 ng / g / g PCB153 was 6.04ng / g / g / 180 and 6.86 ng / g / 180, respectively. The results showed that. PCBs levels in the blood and semen of infertile patients were significantly higher than the normal average. By comparing the concentration of PCBs in blood and semen of infertile patients and normal people, it was found that the concentration of PCBs in blood and semen of infertile patients was higher. The factors influencing the concentration of PCBs were occupation. BMI, age, etc. The concentration of PCBs in blood and semen of self-employed people is higher than that in IT industry. The concentration of PCBs in semen of patients living in urban areas is higher than that of patients living in suburbs. On the other hand, people with moderate PCBs in the blood had lower PCBs concentration, too light or too heavy, which would lead to higher PCBs concentration. The study also found that the older the patient was, the lower the total PCBs concentration in blood and semen was. At present, the research on the relationship between PCBs and male reproductive health at home and abroad is still in the exploratory stage. This study provides data support for the epidemiological study in this field and reproductive health problems caused by environmental hormones.
【学位授予单位】：华南理工大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R114;X503.1

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