华南地区食品中非O157致泻大肠杆菌分布特点及分型研究

发布时间：2018-03-04 22:04

本文选题：食品　切入点：致泻大肠杆菌　出处：《现代食品科技》2017年03期 　论文类型：期刊论文

【摘要】：为深入了解华南地区食品中非O157致泻大肠杆菌(DEC)的污染分布情况和特点,本研究随机采集了该地区12个城市的食品样品,参考GB/T 4789.36-2003方法进行检测,并利用多重PCR对DEC进行了分子鉴定。另外,分别采用MLST分型方法和药敏纸片法对DEC菌株的遗传特性和耐药性进行了分析。结果表明,1000份样品中有164份检出DEC,总污染率高达16.4%。肉类和水产品污染较严重。在五种DEC中,EPEC检出率最高(8.0%),其次为ETEC(6.2%),EIEC(3.4%)和STEC(0.4%)。样品中共分离到207株DEC。MLST分型产生了58种ST型,其中44个为已报道的ST型,14个为数据库中新的ST型。聚类分析表明这些菌株共有8个克隆复合物(CC),CC10为其中最大的一个。DEC菌株对四环素、复方新诺明、氨苄西林、头孢噻吩和氯霉素等具有高抗性。有关部门应加强非O157致泻大肠杆菌的监控,减少食源性疾病的发生。
[Abstract]:In order to understand the distribution and characteristics of non-O157-induced Escherichia coli contamination in food in South China, food samples from 12 cities in this area were randomly collected and detected by GB/T 4789.36-2003. The molecular identification of DEC was carried out by multiplex PCR. The genetic characteristics and drug resistance of DEC strains were analyzed by MLST typing method and drug sensitive disk method. The results showed that 164 out of 1 000 samples were detected, the total contamination rate was as high as 16. 4%. The contamination of meat and aquatic products was serious. The detection rate of EECs was the highest (8.0%), followed by ETEC6.2and EIEC3.4) and STEC0.4The samples were isolated from 207 strains of DEC.MLST and 58 ST-types were found. Among them, 44 were reported ST-type, 14 were new ST-forms in the database. Cluster analysis showed that these strains had 8 clone complex CC10 as one of the largest. DEC strains were tetracycline, compound sulfamine, ampicillin. Cefthiophene and chloramphenicol have high resistance. The departments concerned should strengthen the surveillance of non-O157-induced Escherichia coli and reduce the incidence of foodborne diseases.
【作者单位】：广东省微生物研究所省部共建华南应用微生物国家重点实验室广东省菌种保藏与应用重点实验室广东省微生物应用新技术公共实验室;
【基金】：国家国际科技合作专项(2013BAD,16B05) 广东省科技计划项目(2014B050504007)
【分类号】：R155.5

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