正己烷对人外周血淋巴细胞DNA损伤的人群调查及神经行为功能测试研究

发布时间：2018-03-05 07:45

本文选题：正己烷　切入点：淋巴细胞　出处：《浙江大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的用γH2AX识别抗体流式细胞术检测接触正已烷作业工人对人外周血淋巴细胞DNA的损伤,探讨正已烷的遗传毒性。用中文计算机化神经行为测试软件检测接触正已烷作业工人神经行为功能,探讨和验证低浓度职业正已烷接触致工人神经系统隐匿性损害的可能性,及神经行为功能测试反映神经系统早期损伤的可行性。方法提取某印刷公司接触正已烷工人33例(接触组)和该公司未接触正已烷工人17例(内对照组)及某非职业接触有害因素居民52例(外对照组)的外周血淋巴细胞,采用FCM法检测淋巴细胞DNA损伤情况。同时,从该工厂选择32名接触正已烷的工人(接触组)与32名未接触正已烷的工人(对照组),对两组进行神经行为功能测试。结果接触组、内对照组、外对照组DNA损伤率均数差异有统计学意义(P0.001),进一步分析,结果可见,接触组DNA损伤率(14.30±15.08)%高于内对照组(6.01±3.25)%,差异有统计学意义(P0.05),接触组DNA损伤率高于外对照组(0.32±0.45)%,差异有统计学意义(P0.01)；内对照组DNA损伤率高于外对照组,差异有统计学意义(P0.01)。同时接触组、内对照组和外对照组的几何荧光强度差异有统计学意义(P0.01),经两两比较,接触组几何平均荧光强度(1.51±0.87)FIU高于内对照组(1.04±0.23)FIU,差异有统计学意义(P0.05),接触组几何平均荧光强度高于外对照组(0.73±0.28)FIU,差异有统计学意义(P0.01)；内对照组几何平均荧光强度高于外对照组,差异有统计学意义(P0.01)。神经行为功能测试结果：接触组消极情绪(紧张-焦虑、抑郁-沮丧、愤怒-敌意、疲惫-惰性、困惑-迷茫)得分高于对照组,差异有统计学意义(P0.05)。接触组心算平均准确数、心算能力平均指数、听数字广度平均准确数、听数字广度能力指数、曲线吻合平均准确数、曲线吻合平均能力指数、目标追踪平均准确数均低于对照组,差异有统计学意义(P0.05)。结论职业正已烷接触可致人外周血淋巴细胞DNA损伤,正已烷具有一定的DNA损伤效应；同时利用FCM检测职业接触正已烷工人的DNA损伤具有可行性。长期接触低浓度正已烷对神经行为功能有一定损伤作用。计算机化神经行为功能测试软件在检测神经系统隐匿性损伤方面具有一定的敏感性。
[Abstract]:Objective to detect the DNA damage of human peripheral blood lymphocytes in workers exposed to n-hexane by using 纬 H _ 2AX recognition antibody flow cytometry. To investigate the genotoxicity of n-hexane. To detect the neurobehavioral function of workers exposed to n-hexane by computerized neurobehavioral test software in Chinese, and to explore and verify the possibility of occult damage to the nervous system of workers exposed to n-hexane at low concentration. Methods 33 workers exposed to n-hexane (exposure group), 17 workers not exposed to n-hexane (internal control group) and 17 non-hexane workers (internal control group) were extracted from a printing company. Peripheral blood lymphocytes in 52 cases of occupational exposure to harmful factors (external control group), FCM method was used to detect DNA damage in lymphocytes. 32 workers exposed to n-hexane (exposure group) and 32 workers without n-hexane exposure (control group) were selected from the factory to test the neurobehavioral function of the two groups. There was significant difference in the mean of DNA damage rate between the internal control group and the external control group. The DNA damage rate in the exposed group was 14.30 卤15.08% higher than that in the internal control group (6.01 卤3.25), the difference was statistically significant (P 0.05), the DNA damage rate in the exposure group was higher than that in the external control group (0.32 卤0.45), the difference was statistically significant (P 0.01), and the DNA damage rate in the internal control group was higher than that in the external control group (P 0.01). The difference of geometric fluorescence intensity between the internal control group and the external control group was statistically significant (P 0.01). The geometric mean fluorescence intensity of the exposed group was 1.51 卤0.87 FIU higher than that of the internal control group (1.04 卤0.23) FIUs, the difference was statistically significant (P 0.05), the geometric mean fluorescence intensity of the contact group was higher than that of the external control group (0.73 卤0.28) FIUs, the difference was statistically significant (P0.01), the geometric mean fluorescence intensity of the internal control group was higher than that of the external control group, and the geometric mean fluorescence intensity of the contact group was higher than that of the external control group. The difference was statistically significant (P 0.01). The scores of negative emotions (tension and anxiety, depression and depression, anger and hostility, fatigue and inertia, bewilderment and confusion) in the contact group were higher than those in the control group. The difference was statistically significant (P 0.05). In the contact group, the average number of mental arithmetic accuracy, the average index of mental arithmetic ability, the average accuracy of hearing digit span, the index of hearing digital span ability, the average accuracy of curve anastomosis, the average ability index of curve anastomosis, the average accuracy of hearing digit span, the average ability index of curve coincidence, The average accurate number of target tracing was lower than that of the control group (P 0.05). Conclusion Occupational n-hexane exposure can cause DNA damage in human peripheral blood lymphocytes, and n-hexane has a certain DNA damage effect. At the same time, it is feasible to use FCM to detect DNA damage in workers exposed to n-hexane. Long-term exposure to low concentration n-hexane has a certain effect on neurobehavioral function. Computerized Neurobehavioral function Test Software is used to detect nerve function. The system is sensitive to occult injury.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R131

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