丁酸钠对饮食诱导肥胖大鼠的作用

发布时间：2018-03-06 08:47

本文选题：丁酸钠　切入点：高脂饲料　出处：《浙江大学学报(农业与生命科学版)》2015年02期 　论文类型：期刊论文

【摘要】：为建立由高脂饲料诱导的肥胖易感(obesity-susceptible,OS)和肥胖抵抗(obesity-resistant,OR)大鼠模型,并探讨在高脂饲料中添加丁酸钠对二者的作用,给予SD(Sprague-Dawley)大鼠高脂饲料饲喂3周后依据其体质量增加量排序并再分组,上1/3作为OS组,下1/3作为OR组,继续给予高脂饲料喂养12周,OS大鼠体质量增加明显,体质指数、皮下脂肪、内脏脂肪和体脂肪含量以及血清总胆固醇、甘油三酯、低密度脂蛋白、瘦素与对照组比较显著增高,差异有统计学意义(P0.05),OR组各项指标与对照组比较在统计学上无显著差异(P0.05).按照此造模方法从饲喂高脂饲料第4周起,丁酸钠干预(obesity intervention with sodium butyrate,OI)组增加8 mmol/L丁酸钠生理盐水(0.9%氯化钠)溶液1 m L灌胃,1次/日;肥胖对照(obesity control,OC)组则用1 m L 0.9%氯化钠溶液灌胃,1次/日,共干预16周.结果表明:添加丁酸钠后,OI组大鼠体质量增加、体质指数、皮下脂肪、内脏脂肪和体脂肪含量以及血清总胆固醇、甘油三酯、低密度脂蛋白、瘦素与OC组比较均显著改善,差异有统计学意义(P0.05).说明SD大鼠对于高脂饲料具有不同的应答能力,可获得肥胖敏感及肥胖抵抗模型;对于高脂饮食敏感的SD大鼠,在其高脂饲料中添加丁酸盐能够改善其体质量增加与体质指数,减少体脂肪含量,降低血脂与血清瘦素水平.
[Abstract]:In order to establish a rat model of obesity susceptible to obesity induced by high fat diet, and to explore the effects of adding sodium butyrate to high fat diet, the rat models of obesity resistance and obesity-resistance were established. The rats were fed with high fat diet for 3 weeks. The rats were divided into two groups according to their increased body weight. The upper 1/3 as OS group and the lower 1/3 as OR group, the body mass increased significantly, body mass index (BMI) and subcutaneous fat increased after 12 weeks of high fat diet feeding. The contents of visceral fat and body fat, serum total cholesterol, triglyceride, low density lipoprotein and leptin were significantly higher than those in the control group. There was no statistically significant difference in the indexes of the OR group compared with the control group (P 0.05). According to this method, the model was made from the 4th week after feeding high fat feed. In the butyrate intervention with sodium butyrate Oi group, 8 mmol/L sodium butyrate saline 0.9% sodium chloride solution was added to the stomach once a day, while the obese control group was given 1 mL sodium chloride solution once a day, while the obese control group was given 1 mL 0.9% sodium chloride solution once a day. The results showed that the body mass, body mass index, subcutaneous fat, visceral fat and body fat, serum total cholesterol, triglyceride, low density lipoprotein (LDL) and serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL) were increased in the OI group after treatment with sodium butyrate for 16 weeks. Leptin was significantly improved compared with OC group, and the difference was statistically significant (P 0.05). The results showed that SD rats had different response ability to high fat diet and could obtain obesity sensitive and obesity resistant model, and SD rats were sensitive to high fat diet. The addition of butyrate to its high fat diet could improve the body mass and body mass index, decrease the body fat content, and decrease the serum leptin and lipid levels.
【作者单位】：华中农业大学食品科学技术学院环境食品学教育部重点实验室;武汉市普爱医院;
【基金】：中央高校基本科研业务费专项资金(2011QC073) 湖北省自然科学基金(2013CFB197)
【分类号】：R151

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