动物性食品中环境雌激素残留的检测与分析

发布时间：2018-03-06 09:24

本文选题：环境雌激素　切入点：重组基因酵母　出处：《华中科技大学》2013年硕士论文　论文类型：学位论文

【摘要】：目的：随着工农业的发展，环境雌激素在我们的生活中被广泛应用，经研究发现，它们可以干扰人类和动物的内分泌系统，引起发育、行为和生殖等各方面的异常变化，这严重影响了人类的健康。食品是人类生存和发展的物质基础，是人类暴露于环境雌激素的主要途径。因此，，开展食品中环境雌激素的监测，对于发现和消除对人类健康的潜在危害具有重要意义。方法：本研究采用重组基因酵母筛检法（YES）对中国四个城市居民经常食用的16种142个动物性食品的雌激素活性进行评价，并结合高效液相色谱-串联质谱法（LC-MS/MS）分析样品中常见8种环境雌激素的含量。结果：主要内容包括两部分：第一部分：动物性食品中总的雌激素活性测定本部分采用该方法对动物性食品中总的雌激素活性进行测定。结果表明所有样品均检测到一定的雌激素活性，生物雌二醇当量浓度（EEQbio）在8.29~118.32ng/g之间。四大类动物性食品中，家畜样品测得的雌激素活性值最高，EEQbio为34.28~118.32ng/g，比鸡蛋测得值高4~5倍，鸡蛋样品测得的雌激素活性值最低，EEQbio为8.29~20.91ng/g；家禽、水产品测得的EEQbio的范围在12.82~110.94ng/g之间。第二部分：动物性食品中8种常见环境雌激素含量的测定采用高效液相色谱-串联质谱方法（LC/MS/MS）检测了样品中雌酮（E1）、17β-雌二醇（E2）、雌三醇（E3）、乙炔基雌二醇（EE2）、己烯雌酚（DES）、双酚A（BPA）、壬基酚（4-NP）、辛基酚（4-t-OP）等8种常见的环境雌激素。结果表明，天然雌激素E1、E3在动物性食品中均未检出，所有的样品中都检测到E2，检出率达100%，其浓度范围在0.44~15.04ng/g之间，中值浓度为7.93ng/g。人工合成雌激素EE2在部分样品中有检出，总体检出率为33.1%，在样品中检测到的最高浓度为2.8ng/g，DES在任何样品中均未检测到。酚类环境雌激素BPA和4-NP有较高的检出率约83%，4-t-OP的检测值均低于检测下限。BPA和4-NP的浓度范围在nd~35.76ng/g之间。结论：重组基因酵母筛检法是筛选和定量分析样品总雌激素活性的一种快速、有效的方法，重组基因酵母对雌激素类化合物有较高的灵敏度。所有样品测得的EEQbio（8.29-118.32ng/g）均高于化学雌二醇当量（EEQchem，0.44-15.91ng/g），两种方法测得的EEQ值有一定的相关性（r=0.84）。
[Abstract]:Objective: with the development of industry and agriculture, environmental estrogens are widely used in our daily life. It has been found that they can disturb the endocrine system of humans and animals and cause abnormal changes in development, behavior and reproduction. Food is the material foundation of human survival and development and the main way of human exposure to environmental estrogens. Therefore, the monitoring of environmental estrogens in food is carried out. It is of great significance to discover and eliminate the potential harm to human health. Methods: in this study, the estrogenic activity of 16 kinds of 142 kinds of animal food frequently consumed by four cities in China was evaluated by recombinant gene yeast screening method (YESs). The contents of 8 common environmental estrogens in the samples were analyzed by HPLC-MS-MS. Results: the main content consists of two parts:. Part one: determination of total estrogen activity in animal foods. In this part, the total estrogenic activity in animal food was determined by this method. The results showed that all samples had certain estrogenic activity, and the biological equivalent concentration of estradiol was between 8.29 and 118.32 ng / g. The estradiol activity of domestic animal samples was 34.28 ~ 118.32ng / g, which was 4 ~ 5 times higher than that of eggs. The lowest estrogenic activity of egg samples was 8.29 ~ 20.91ng / g, and the range of EEQbio measured by poultry and aquatic products was 12.82110.94ng / g. Part II: determination of eight common environmental estrogens in animal foods. Eight common environmental estrogens were detected by HPLC / MS / MS / MS / MS / MS, including estradiol E1O17 尾 -estradiol (E2N), estriol (E3N), ethylestradiol (E2N), diethylstilbestrol (DESN), bisphenol A (BPAA), nonylphenol 4-NPand octylphenol (4-t-OPP), and so on. No natural estrogen E _ 1C _ 3 was detected in animal food, and E _ 2 was detected in all samples, and the detection rate was 100%. The concentration range was between 0.44 ~ 15.04 ng / g, and the median concentration was 7.93 ng / g. Synthetic estrogen EE2 was detected in some samples. The total detectable rate was 33.1. The highest concentration of 2.8ng / g des was not detected in any sample. The detection rates of phenolic environmental estrogens BPA and 4-NP were higher than those of the lower limit of detection. BPA and 4-NP were in the range of nd~35.76ng/g. Conclusion: the screening method of recombinant gene yeast is a rapid and effective method for screening and quantitative analysis of total estrogen activity of samples. The sensitivity of recombinant gene yeast to estrogen compounds was higher than that of chemical estradiol equivalent EEQbiotin (8.29-118.32ng / g), and the EEQ values measured by the two methods had a certain correlation with 0.84 ng / g ~ (-1) of estradiol equivalent (Eestradiol) = 0.44-15.91ng / g ~ (-1) 路g ~ (-1) 路g ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1)).
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R155.5

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