两种方法检测水中总大肠菌群和大肠埃希菌的等效性分析

发布时间：2018-03-10 08:11

本文选题：总大肠菌群　切入点：大肠埃希菌　出处：《卫生研究》2017年05期 　论文类型：期刊论文

【摘要】：目的比较实验室自制酶底物滤膜测试片法和51孔定量盘酶底物法在检测水中总大肠菌群与大肠埃希菌上是否具有等效性。方法选取不同地区的3个实验室,分别采集生活饮用水和水源水,同时采用酶底物滤膜测试片法和51孔定量盘酶底物法进行总大肠菌群和大肠埃希菌检测,按照ISO 17994∶2014《水质-微生物方法之间的等效性建立标准》对检测结果数据进行分析。结果 3个实验室采用两种方法检测总大肠菌群均数的扩展不确定度的置信上限范围为(0.0494,0.0903),置信下限范围为(-0.0713,-0.0490);检测大肠埃希菌均数的扩展不确定度的置信上限范围为(0.0307,0.0882),置信下限范围为(-0.0928,-0.0381);两者置信上限和下限均在可接受的区间范围内(0,0.10000)和(-0.1000,0)。说明3个实验室采用两种方法检测总大肠菌群与大肠埃希菌的等效性验证结果均无差异。结论酶底物滤膜测试片法和51孔定量盘酶底物法在检测总大肠菌群与大肠埃希菌参数上具有等效性,进行总大肠菌群与大肠埃希菌检测时,酶底物滤膜测试片法可以作为51孔定量盘酶底物法的替代和补充。
[Abstract]:Objective to compare the equivalence of enzyme substrate filter membrane test method and 51 hole quantitative enzyme substrate method in the detection of total coliform bacteria and Escherichia coli in water. Methods three laboratories in different regions were selected. Drinking water and source water were collected, and total coliform bacteria and Escherichia coli were detected by enzyme substrate method and 51 hole quantitative enzyme substrate method. According to ISO 17994: 2014, "criteria for establishing equivalence between Water quality and microbial methods", the data of test results were analyzed. Results the confidence upper limit of extended uncertainty of testing the mean number of total coliform bacteria by two methods was used in three laboratories. The range of confidence limits is 0.0494-0.0903, the lower confidence limit is 0.0713- 0.04900.The confidence upper limit of the extended uncertainty for the detection of Escherichia coli is 0.0307 0.0882, and the lower confidence limit is 0.0928- 0.0381g; the confidence upper and lower limits are both within the acceptable range of 0.10000 and 0.10000.100.The results show that the confidence upper and lower limits are within the acceptable range. There was no difference in the results of the equivalence between the two methods for the detection of total coliform bacteria and Escherichia coli in three laboratories. Conclusion the method of enzyme substrate filter membrane test and 51 hole quantitative diskette substrate method are used to detect total coliform bacteria and Escherichia coli. The parameters of the bacteria are equivalent. In the detection of total coliform and Escherichia coli, the enzyme substrate filter membrane method can be used as the substitute and supplement of 51 hole quantitative enzyme substrate method.
【作者单位】：中国疾病预防控制中心环境与健康相关产品安全所;辽宁省鞍山市疾病预防控制中心;河南省西平县疾病预防控制中心;河南省尉氏县疾病预防控制中心;
【基金】：国家卫生和计划生育委员会公益性行业专项课题(No.20132004-1-2-1)
【分类号】：R123.1

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