ELISA检测小麦粉中脱氧雪腐镰刀菌烯醇的优化

发布时间：2018-03-11 10:22

本文选题：脱氧雪腐镰刀菌烯醇　切入点：酶联免疫吸附实验　出处：《现代预防医学》2015年03期 　论文类型：期刊论文

【摘要】：目的针对ELISA法检测脱氧雪腐镰刀菌烯醇(DON),对小麦粉样品中DON提取和DON标准工作液制备进行优化。方法以超声提取法提取小麦粉样品中的DON,以含10%甲醇的DON阴性样品提取液作DON的ELISA标准工作液溶解相,对检测方法进行优化,并以优化的方法对50份小麦粉样品进行检测。结果检测方法经优化后,样品经超声提取仅需10 min,2 500.00 ng/g、500.00 ng/g、100.00 ng/g 3个浓度水平的平均回收率为94.69%~102.71%,平均CV%为6.50%,50份小麦粉样品DON检出率为30.00%,DON污染水平在57.26~112.37μg/kg,中位数为84.12μg/kg,所有样品中DON含量均未超过国家规定的限量标准。结论优化后的检测方法,大大缩短了样品提取时间,提高了样品提取率,降低了样品基质的干扰,方法重复性良好。
[Abstract]:Objective to detect the deoxynivalenoldol in wheat flour by ELISA, to optimize the extraction of DON from wheat flour and the preparation of standard working solution of DON. Methods the method of ultrasonic extraction was used to extract DON from wheat flour, and the negative sample of DON containing 10% methanol was used to extract DON from wheat flour. The product extract was used as the ELISA standard working liquid dissolution phase of DON. The detection method was optimized, and 50 wheat flour samples were detected by the optimized method. The average recovery of the three concentrations of 100.00 ng/g was 94.69 ~ 102.71, and the detection rate of DON in 50 wheat flour samples was 6.50%. The detection rate of DON was 30.002. 00 渭 g / kg at 57.26112.37 渭 g / kg, median 84.12 渭 g / kg, and the DON content in all samples was not higher than that in the country, and the average recovery was 94.69% 102.71%, and the average CV% was 6.50%. The detection rate of DON in 50 wheat flour samples was 30.0026112.37 渭 g / kg, and the median was 84.12 渭 g / kg. Standard for limited limits established by the United Nations. Conclusion the optimized detection method, The sample extraction time was shortened, the extraction rate was increased, the interference of sample matrix was reduced, and the method was reproducible.
【作者单位】：济南铁路疾病预防控制中心微生物检验科;
【分类号】：R155.5

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