PM2.5对支气管上皮细胞中VEGF、ROS的影响

发布时间：2018-03-11 11:20

本文选题：PM.　切入点：支气管　出处：《中国现代医学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的探讨细颗粒物(PM2.5)对支气管上皮细胞中血管内皮生长因子(VEGF)、活性氧类(ROS)的影响。方法培养支气管上皮细胞16HBE,分为4组:生理盐水对照组、25μg/ml PM2.5组、50μg/ml PM2.5组和100μg/ml PM2.5组,测定各组细胞增殖活性、ROS及VEGF表达水平。结果 50μg/ml PM2.5组和100μg/ml PM2.5组细胞增殖活性分别为(89.80±5.85)%和(77.20±7.70)%,低于生理盐水对照组(P0.05);50μg/ml PM2.5组和100μg/ml PM2.5组染毒12和24 h ROS均高于生理盐水对照组(P0.05),其中100μg/ml PM2.5组染毒12和24 h ROS水平均最高,分别为(16.60±0.40)和(24.01±0.55);50μg/ml PM2.5组和100μg/ml PM2.5组VEGF蛋白表达高于生理盐水对照组(P0.05),其中100μg/ml PM2.5组VEGF蛋白表达水平最高(0.892±0.028)。结论 PM2.5能对支气管上皮细胞造成氧化损伤,上调VEGF蛋白表达。
[Abstract]:Objective to investigate the effect of PM2.5 on vascular endothelial growth factor (VEGF) in bronchial epithelial cells (BECs). Methods 16 HBEs were cultured in bronchial epithelial cells and divided into 4 groups: normal saline control group, 25 渭 g / ml PM2.5 group, 50 渭 g / ml PM2.5 group and 100 渭 g / ml PM2.5 group. Results the proliferative activity of Ros and VEGF in 50 渭 g / ml PM2.5 group and 100 渭 g / ml PM2.5 group were 89.80 卤5.85% and 77.20 卤7.70%, respectively, which were lower than those in normal saline control group (P 0.05 渭 g / ml PM2.5 group and 100 渭 g / ml PM2.5 group at 12 and 24 h). The control group (P 0.05) was exposed to 100 渭 g / ml PM2.5 for 12 h and 24 h, and the ROS level was the highest in 100 渭 g / ml PM2.5 group. The expression of VEGF protein in 50 渭 g / ml PM2.5 group and 100 渭 g / ml PM2.5 group was higher than that in normal saline control group (P 0.05). The highest expression level of VEGF protein in 100 渭 g / ml PM2.5 group was 0.892 卤0.028 渭 g / ml. Conclusion PM2.5 can cause oxidative damage to bronchial epithelial cells and up-regulate the expression of VEGF protein.
【作者单位】：湖北省武汉市儿童医院;湖北医药学院附属太和医院;
【基金】：武汉市科学技术计划项目(No:2015061701011632)
【分类号】：R12

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