食蟹猴腹腔注射低剂量百草枯致胰腺α-突触核蛋白的病理变化

发布时间：2018-03-12 06:49

本文选题：食蟹猴　切入点：百草枯　出处：《基因组学与应用生物学》2017年05期 　论文类型：期刊论文

【摘要】：本研究旨在通过食蟹猴经腹腔注射低剂量百草枯来探讨胰腺α-突触核蛋白(α-synuclein,α-syn)的病理变化。由广西南宁灵康赛诺科生物科技有限公司提供的12只(8~10岁)健康雄性食蟹猴为实验动物,随机分为实验组A、B和正常对照组C,每组4只,实验组分别于给药百草枯(paraquat,PQ)5个月,2个月后安乐死(对照组每阶段2只)。利用免疫组织化学方法和免疫蛋白印记方法检测各组食蟹猴胰岛α-syn的表达特点和表达量。结果显示,α-syn免疫活性物质主要见于胰岛细胞胞浆,并且α-syn在各组食蟹猴胰岛存在差异表达,其中给药百草枯5个月后的食蟹猴胰岛α-syn表达量最高,对照组表达量最低。我们的研究表明食蟹猴腹腔注射低剂量百草枯可致胰岛α-syn表达上调,百草枯是造成胰岛腺α-syn差异表达的关键因素之一,其结论将为相关疾病的模型制作、早期诊断及干预提供新的思路。
[Abstract]:The aim of this study was to investigate the pathological changes of 伪 -synuclein (伪 -synn) in pancreas by intraperitoneal injection of paraquat into the abdominal cavity of crab-eating monkeys. 12 healthy male food samples were provided by Guangxi Nanning Ling Kangsanoki Biotechnology Co., Ltd. The crab monkey is an experimental animal. The rats were randomly divided into two groups: experimental group (n = 4) and control group (n = 4). The experimental group was treated with paraquat PQ for 5 months. After 2 months of euthanasia (control group, 2 rats in each stage), the expression of 伪 -syn in pancreatic islets of each group was detected by immunohistochemical method and immunoglobulin imprinting method. The results showed that 伪 -syn immunoreactive substances were mainly found in the cytoplasm of islet cells. The expression of 伪 -syn in the islets of crab-eating monkeys was the highest after 5 months of administration of paraquat, and the expression of 伪 -syn in the islets of crab-eating monkeys was the highest. Our study showed that the expression of 伪 -syn in pancreatic islets was up-regulated by intraperitoneal injection of paraquat, and paraquat was one of the key factors causing the differential expression of 伪 -syn in pancreatic islets. Early diagnosis and intervention provide new ideas.
【作者单位】：广西医科大学转化医学研究中心长寿与老年相关疾病省部共建教育部重点实验室;广西南宁灵康赛诺科生物科技有限公司;首都医科大学宣武医院神经生物学研究室;
【基金】：国家高技术研究发展计划(2012AA02A514) 国家重点基础研究发展计划(2011CB504101) 广西区科技厅项目(1598025-31) 南宁市科技局项目(20155192,20163349)共同资助
【分类号】：R114

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