乳制品中四种病原菌多重荧光PCR检测方法的建立与应用

发布时间：2018-03-12 11:05

本文选题：多重荧光PCR　切入点：阪崎肠杆菌　出处：《基因组学与应用生物学》2017年09期 　论文类型：期刊论文

【摘要】：本研究建立了可快速特异检测乳制品中阪崎肠杆菌、沙门氏菌、金黄色葡萄球菌和单核细胞增生李斯特氏菌的多重荧光PCR方法。通过设计阪崎肠杆菌、沙门氏菌、金黄色葡萄球菌和单核细胞增生李斯特氏菌的特异性引物和探针,建立了多重PCR反应体系,评价了该方法的特异性、灵敏度和精密度,并用该方法和国标法分别检测了150份乳制品中的4种致病菌,对检测结果进行对比。结果显示,检测目标菌为相应阳性,检测其他12种非目标供试菌为阴性;检测灵敏度分别为1 cfu/μL;其精密度的变异系数均小于5%;与国标检测方法相比其检测结果具有良好的一致性。
[Abstract]:A multiplex fluorescence PCR method was developed for the rapid and specific detection of Enterobacter sakazakii, Salmonella, Staphylococcus aureus and Listeria monocytogenes in dairy products. The specific primers and probes of Staphylococcus aureus and Listeria monocytogenes were used to establish a multiplex PCR reaction system, and the specificity, sensitivity and precision of the method were evaluated. Four pathogenic bacteria in 150 samples of dairy products were detected by this method and the national standard method. The results showed that the target bacteria were positive and the other 12 non-target bacteria were negative. The detection sensitivity is 1 cfu/ 渭 L, and the coefficient of variation of the precision is less than 5 渭 L, which is consistent with the national standard detection method.
【作者单位】：上海震旦学院公共卫生与护理学院;上海出入境检验检疫局;江苏同科医药科技有限公司;
【基金】：上海市科委技术标准课题(13DZ05022702) 上海市晨光计划(13CGB25)共同资助
【分类号】：R155.5

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