甲醛对HepG2细胞凋亡的影响

发布时间：2018-03-13 05:20

本文选题：甲醛　切入点：HepG细胞　出处：《毒理学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的探讨甲醛对HepG2细胞凋亡的影响。方法以0.004、0.02、0.1、0.5、2.5、12.5 mmol/L的甲醛(formaldehyde)分别处理人肝癌HepG2细胞24和48 h,采用MTT法检测细胞活性。分别以0.004、0.02、0.1 mmol/L的甲醛处理人肝癌HepG2细胞24和48 h,采用Western blot法检测p53、mdm2、Caspase-3、RIP1、RIP3和NF-κB的蛋白表达水平。结果与阴性对照组相比较,0.5~12.5 mmol/L FA染毒24和48 h可显著降低HepG2细胞活性(P0.05);染毒24 h后Caspase-3蛋白表达降低(P0.05);p53、p-p53、mdm2、RIP3表达水平在染毒24和48 h后均明显降低(P0.05);cleaved RIP1表达水平在0.1 mmol/L剂量染毒24和48 h后表达增加(P0.05)上述变化均有统计学意义;染毒24和48 h后NF-κB蛋白表达无明显变化。结论甲醛可能是通过死亡受体途径而不是线粒体途径引起肝细胞凋亡。
[Abstract]:Objective to investigate the effect of formaldehyde on apoptosis of HepG2 cells. Methods Human hepatoma HepG2 cells were treated with 0. 004 (0. 021) and 2. 512.5 mmol/L formaldehyde for 24 h and 48 h, respectively. The cell activity was detected by MTT assay, and the cells were treated with 0. 004 ~ 0. 02 mmol/L formaldehyde for 24 and 48 h, respectively. Western blot assay was used to detect the protein expression of RIP3 and NF- 魏 B. results compared with the negative control group, the expression of Caspase-3 protein decreased significantly at 24 and 48 hours after exposure to 0.5 and 12.5 mmol/L FA, and the expression of Caspase-3 protein decreased in 24 h after exposure to P0.05mdm2mdm2mdm2mdm2mdm2mdm2mdm2mdm2mdm2mdm2mdm2mdm2mdm2rp3 after 24 h exposure to P0.05mdm2mdm2RIP3 after 24 h of exposure to P0.05mdm2mdm2rp3. And 48 h after treatment, the expression level of P0.05Cleaved RIP1 was significantly decreased. The expression of P0.05 was increased 24 h and 48 h after exposure to 0.1 mmol/L). The expression of NF- 魏 B protein did not change after 24 and 48 h exposure. Conclusion formaldehyde may induce apoptosis of hepatocytes through death receptor pathway rather than mitochondria pathway.
【作者单位】：山西医科大学公共卫生学院环境卫生教研室;
【基金】：山西省留学回国人员科技活动择优资助项目(2014-95) 山西省自然科学基金(2013011052-1)
【分类号】：R114

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