甲基汞通过miRNA对人胚胎神经干细胞细胞周期相关基因表达的调控

发布时间：2018-03-13 21:18

本文选题：人胚胎神经干细胞　切入点：甲基汞　出处：《环境与职业医学》2015年05期 　论文类型：期刊论文

【摘要】：[目的]通过研究甲基汞对人胚胎神经干细胞mi RNA表达的影响,探讨低剂量甲基汞对神经干细胞细胞周期调控基因的调节作用。[方法]以0、10、50 nmol/L甲基汞染毒人胚胎神经干细胞24 h后,用MTT法测定甲基汞对细胞活力影响,应用逆转录多聚酶链反应(RT-PCR)检测甲基汞对细胞周期调控基因(p16、p21)的m RNA的表达水平影响,利用实时荧光定量多聚酶链反应技术检测调控p16、p21的mi RNA(mi R-24、mi R-106a)的表达情况。[结果]50 nmol/L甲基汞染毒组细胞活力降低为对照组的53.5%,差异有统计学意义(P0.05);p16与p21基因的m RNA表达水平随着甲基汞染毒浓度的升高而升高,差异均有统计学意义(P0.05),但10 nmol/L与50 nmol/L组的p16基因表达差异无统计学意义。mi R-24、mi R-106a的表达水平随着甲基汞染毒浓度的升高而降低,差异有统计学意义(P0.05)。[结论]50 nmol/L的甲基汞可以引起人胚胎神经干细胞增殖抑制,并可能通过mi RNA调节细胞周期调控基因的表达。
[Abstract]:[objective] to study the effect of methylmercury on the expression of mi RNA in human embryonic neural stem cells. To investigate the regulatory effect of low dose methylmercury on cell cycle regulation gene of neural stem cells. [methods] the effects of methylmercury on cell viability were determined by MTT assay after exposure to human embryonic neural stem cells (NSCs) for 24 h. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the effect of methylmercury on the expression of m RNA in cell cycle regulatory gene p16 p21. Real-time fluorescence quantitative polymerase chain reaction (RQPCR) was used to detect the expression of the regulated mi RNA(mi R-24mMIR-106a of p16 p21. [results] the cell viability of 50 nmol/L methylmercury exposed group was reduced to 53.5% of that of the control group, and the difference was statistically significant (P 0.05) and the m RNA of p21 gene was significantly lower than that of the control group. The expression level increases with the increase of methylmercury concentration, There was no significant difference in the expression of p16 gene between 10 nmol/L group and 50 nmol/L group. The expression level of p16 gene decreased with the increase of methylmercury concentration. [conclusion] 50 nmol/L methylmercury can inhibit the proliferation of human embryonic neural stem cells and regulate the expression of cell cycle regulatory genes through mi RNA.
【作者单位】：复旦大学公共卫生学院教育部公共卫生安全重点实验室;
【基金】：国家自然科学基金项目(编号:81472996)
【分类号】：R114

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