16S rDNA克隆文库法分析两种鱿鱼墨多糖对小鼠肠道菌群多样性的影响

发布时间：2018-03-14 00:37

  本文选题：肠道菌群　切入点：鱿鱼墨多糖　出处：《中国食品学报》2016年10期 　论文类型：期刊论文

【摘要】：探讨北太平洋鱿鱼墨多糖与秘鲁鱿鱼墨多糖对化疗模型小鼠肠道菌群的影响。将40只Balb/c小鼠随机分为4组:正常组、模型组、北太平洋鱿鱼墨多糖组、秘鲁鱿鱼墨多糖组。采用环磷酰胺化疗模型,无菌收集小鼠粪便并提取细菌基因组DNA,通过构建各组小鼠肠道细菌的16S rDNA克隆文库,单酶切分型,观察鱿鱼墨多糖对肠道菌群的作用。试验结果表明:两种鱿鱼墨多糖对化疗模型小鼠肠道菌群种类有一定调节作用,且两种鱿鱼墨多糖效果不尽相同。
[Abstract]:To investigate the effects of the ink polysaccharides of squid from North Pacific and Peru on the intestinal flora of chemotherapeutic model mice, 40 Balb/c mice were randomly divided into four groups: normal group, model group, polysaccharide group of squid ink from North Pacific, and so on. Using cyclophosphamide (cyclophosphamide) chemotherapeutic model, the feces of mice were collected aseptically and the genomic DNA of bacteria was extracted. The 16s rDNA clone library of intestinal bacteria in each group was constructed and digested by single enzyme. The effects of squid ink polysaccharides on intestinal flora were observed. The results showed that the two kinds of squid ink polysaccharides could regulate the intestinal flora of chemotherapeutic model mice, and the effects of two kinds of squid ink polysaccharides were different.
【作者单位】： 中国海洋大学食品科学与工程学院;
【基金】：国家自然科学基金项目(31071525) 国家海洋公益性行业科研专项(201105029) “泰山学者”建设工程专项
【分类号】：R151.41
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本文编号：1608840