水源水中贾第鞭毛虫和隐孢子虫的滤膜溶解-免疫磁分离-荧光染色检测方法

发布时间：2018-03-14 12:58

本文选题：水源水　切入点：隐孢子虫　出处：《环境与健康杂志》2015年12期 　论文类型：期刊论文

【摘要】：目的建立水源水中贾第鞭毛虫和隐孢子虫的滤膜溶解-免疫磁分离-荧光染色检测方法。方法水样经混合乙酸纤维膜过滤后,溶解于丙酮,离心浓缩,用免疫磁珠分选浓缩产物中的贾第鞭毛虫和隐孢子虫,并用免疫荧光染色剂染色计数。结果贾第鞭毛虫的平均加标回收率为36.58%,分步加标回收率试验中"富集浓缩"这一步加标回收率最低,为57.04%;隐孢子虫的平均加标回收率为19.58%,分步加标回收率试验中"富集浓缩"这一步的加标回收率最低,为26.55%;二者的平均加标回收率均符合我国GB/T 5750—2006《生活饮用水标准检验方法》中回收率高于10%的要求。结论该方法成本低,操作相对简便,适用于普通实验室对水源水中贾第鞭毛虫和隐孢子虫的检测,其中,"富集浓缩"步骤为影响回收率的关键步骤。
[Abstract]:Objective to establish a filter membrane dissolution-immunomagnetic separation and fluorescence staining method for the detection of Giardia lamblia and Cryptosporidium in source water. Separation of Giardia and Cryptosporidium from concentrated products by immunomagnetic beads, The results showed that the average recovery rate of Giardia lamblia was 36.58, and that of "enrichment concentration" was the lowest. The average standard addition recovery of Cryptosporidium was 19.58, and the recovery rate of enrichment and concentration was the lowest in the stepwise enrichment recovery test. The average recoveries of both of them are in accordance with the requirements of GB/T 5750-2006 Standard Test method for drinking Water, which is higher than 10%. Conclusion this method has low cost and relatively simple operation. It is suitable for the detection of Giardia lamblia and Cryptosporidium in source water in general laboratory, in which the "enrichment and concentration" step is the key step to affect the recovery of Giardia lamblia and Cryptosporidium.
【作者单位】：中国疾病预防控制中心环境与健康相关产品安全所;
【基金】：国家卫生和计划生育委员会公益性行业科研专项(201302004)
【分类号】：R123.1

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