职业性锰接触对工人神经系统多巴胺能神经递质HVA、PRL、Parkin的影响

发布时间：2018-03-14 14:06

  本文选题：职业性锰接触　切入点：UHVA　出处：《山西医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:从多巴胺能神经毒性的角度研究职业性锰接触对职业人群的危害,探讨职业性锰接触对工人机体神经损害的影响。方法:对某钢铁集团修造公司中锰作业环境进行监测,选择该钢铁企业中男性接触锰烟尘危害的电焊工150人作为锰接触组,同时将该钢铁企业的能源动力厂的男性水处理工162人作为对照组。收集各组工人一般情况,如工作制度、年龄、职业工龄史、吸烟等,同时应用精神状态量表(MMSE)、画钟测试(CDT)和语言流畅性方式(VFT)测验锰接触组和对照组工人的神经行为认知功能、各组工人的个人症状与体征,综合测评其神经功能损害程度。依据国家职业卫生标准,监测作业环境空气中锰及其化合物、NOx、O3的含量;测定内暴露剂量尿锰的浓度用石墨炉原子吸收分光光度法。经ELISA试剂盒检测对锰接触和对照组工人尿高香草酸(UHVA)表达水平进行检测,经ELISA试剂盒检测锰接触工人和对照组工人催乳素(PRL)、parkin表达水平。以尿锰分组,比较不同接触组中UHVA、PRL、Parkin表达水平。结果:在锰接触组,其作业场所中的锰及其化合物的浓度(0.120±0.020)、氮氧化物浓度(1.073±0.079)均高于对照组(0.046±0.023、0.919±0.113),有统计学意义(P0.05)。锰接触组VFT(39.90±2.70)及神经行为认知功能测试(72.64±3.11)低于对照组(41.49±2.08;74.51±2.31),有统计学意义(P0.05);且不同接触组之间比较,高接触组中VFT(37.45±1.61、69.68±2.16)及神经行为认知功能测试均低于中、低接触组(40.55±2.54、41.13±2.27;74.36±2.51、73.31±2.73),有统计学意义(P0.05)。锰接触组的个人症状与体征调查中,在乏力、注意力不集中、焦虑、易怒的阳性率(11.3%;12.0%;8.7%;6.7%)均高于对照组(4.9%;5.6%;3.1%;1.9%),均有统计学意义(P0.05)。锰接触组(6.78±1.395)与对照组UHVA水平(4.97±1.242)比较,差异有统计学意义(P0.05),在锰接触组中,不同接触组间UHVA水平比较,中接触组(7.10±1.493)与低工龄(6.37±1.128)和高接触组(6.54±1.279)比较,差异均有统计学意义(P0.01)。锰接触组(12.63±3.108)与对照组(11.10±2.819)的血清PRL表达水平比较,差异有统计学意义(P0.05),锰接触组中不同接触组之间血清PRL表达水平经俩俩比较,中接触组(14.02±2.859)与低工龄(11.56±2.865)、中接触组与高接触组(10.90±2.453)比较,差异均有统计学意义(P0.01)。锰接触组在parkin表达水平(0.559±0.219)上低于对照组(0.639±0.187),有统计学意义(P0.05),锰接触组中不同接触组之间parkin表达水平经俩俩比较,高接触组parkin蛋白表达水平(0.655±0.220)明显低于中、低接触组(0.574±0.212、0.436±0.176),有统计学意义(P0.05)。相关性分析显示,其中神经行为认知功能测试与UHVA、血清PRL表达水平呈负相关关系((rHVA=-0.577,P0.01;(rPRL=-0.394,P0.01),神经行为认知功能测试与Parkin表达水平呈现正相关关系(rparkin=0.675,P0.01)。结论:Ⅰ.职业性锰接触引起了一定的神经行为认知功能改变。Ⅱ.锰接触改变了多巴胺能神经递质表达水平,且随锰接触的时间,影响其神经系统行为认知功能以及多巴胺能神经递质表达的程度。Ⅲ.parkin蛋白表达水平可能作为早期锰中毒的神经毒性指标。
[Abstract]:Objective: from the perspective of dopaminergic neurotoxicity of manganese exposure on occupation occupation harm people, explore the occupation of effects of manganese exposure on human nerve damage to the workers. Methods: monitoring of a steel group company built of manganese in the work environment, the iron and steel enterprises in men exposed to manganese smoke welders 150 as exposed to manganese at the same time, the iron and steel enterprise group, the power plant water treatment workers 162 men as control group were collected. Workers in general, such as working system, age, occupation age history, smoking and so on, at the same time the application of mental state scale (MMSE), clock drawing test (CDT) and verbal fluency (VFT way) test of manganese exposure group and the control group of workers neurobehavioral cognitive function, symptoms and signs of each individual workers, the comprehensive evaluation of the degree of neurological damage. According to the national health standard occupation, work environment monitoring Manganese and its compounds in the air, NOx, O3 content; Determination of exposure dose of manganese in urine by spectrophotometry by graphite furnace atomic absorption. The ELISA kit to detect the contact and control workers urinary homovanillic acid (UHVA) on manganese levels were detected by ELISA kit, detection and control of manganese exposed workers group of workers (PRL), prolactin. The expression level of parkin in urine manganese group, UHVA, compare the different contact group PRL, the expression level of Parkin. Results: in manganese exposure group, the concentration of manganese and its compounds in their workplace in the (0.120 + 0.020), the concentration of nitrogen oxides (1.073 + 0.079) were higher than the control group (0.046 + 0.023,0.919 + 0.113), there was statistical significance (P0.05). VFT manganese exposure group (39.90 + 2.70) cognitive function and neurobehavioral test (72.64 + 3.11) is lower than that of control group (41.49 + 2.08; 74.51 + 2.31), there was statistical significance (P0.05); and the comparison between different contact group, high contact 缁勪腑VFT(37.45卤1.61,69.68卤2.16)鍙婄�炵粡琛屼负璁ょ煡鍔熻兘娴嬭瘯鍧囦綆浜庝福�

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