2015年江西省631位餐饮从业人员带菌状况调查与分子分型研究

发布时间：2018-03-16 18:16

本文选题：餐饮从业人员　切入点：带菌状况　出处：《现代预防医学》2017年19期 　论文类型：期刊论文

【摘要】：目的了解江西省餐饮从业人员的带菌状况并开展分子分型研究。方法对江西省2个监测点79家餐馆和60所学校食堂共631位餐饮从业人员进行了粪便采样。参照国家食品安全风险评估中心提供的方法分别对沙门氏菌、志贺氏菌、致泻性大肠埃希氏菌和诺如病毒进行了检测与分析。采用聚合酶链式反应(PCR)方法、脉冲场凝胶电泳(PFGE)和荧光定量PCR方法分别对致泻性大肠埃希氏菌、沙门氏菌和诺如病毒进行分子分型。结果 631份粪便样本中致泻性大肠埃希氏菌检出率为7.61%,沙门氏菌检出率为2.22%,1份样品中检出诺如病毒,志贺氏菌未检出。致泻性大肠埃希氏菌检出3种不同的血清型,分别为肠聚集性大肠埃希氏菌(EAEC)33株,肠致病性大肠埃希氏菌(EPEC)11株,肠产毒性大肠埃希氏菌(ETEC)4株,检出率分别为5.23%,1.74%,0.06%;14株沙门氏菌的PFGE指纹图谱分为10个型别(相似值100%的为同一PFGE型别);诺如病毒为GI型。结论江西省餐饮从业人员存在携带致病菌的状况,可能引发食源性疾病的暴发,食品监管部门应加强新《食品安全法》的普及,提高从业人员的食品安全卫生意识,降低风险,保证人民群众的身体健康。
[Abstract]:Objective to investigate the bacterial status of catering workers in Jiangxi Province and to study the molecular typing of bacteria. Methods the feces samples were collected from 631 catering workers in 79 restaurants and 60 school canteens in 2 monitoring sites of Jiangxi Province. The home food safety risk assessment center provided methods for salmonella, Shigella, Escherichia coli and Noroviruses were detected and analyzed. The methods of polymerase chain reaction (PCR), pulsed field gel electrophoresis (PFGE) and fluorescence quantitative PCR (PCR) were used to detect and analyze Escherichia coli. Results the detection rate of Escherichia coli in 631 fecal samples was 7.61, and the detection rate of Salmonella was 2.22%. There were 3 different serotypes of Escherichia coli, 33 strains of EAEC, 11 strains of pathogenic Escherichia coli, 4 strains of ETEC, 3 strains of Escherichia coli and 4 strains of enterotoxic Escherichia coli. The PFGE fingerprints of 14 Salmonella strains were divided into 10 types (the same PFGE type with a similar value of 100%, and GI type with a similar value of 100%). Conclusion the restaurant workers in Jiangxi Province have the status of carrying pathogenic bacteria. The food supervision department should strengthen the popularization of the new Food Safety Law, improve the employees' awareness of food safety and hygiene, reduce the risk, and ensure the health of the people.
【作者单位】：江西省疾病预防控制中心;德兴市疾病预防控制中心;乐安县疾病预防控制中心;
【基金】：江西省科技厅科技支撑项目(20141BBG70095) 江西省卫计委科研项目(20166022)(20171465)
【分类号】：R155.7

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