氨基葡萄糖、骨碎补和虾青素对大鼠急性骨关节炎的干预研究

发布时间：2018-03-19 05:30

本文选题：氨基葡萄糖　切入点：骨碎补　出处：《食品工业科技》2017年04期 　论文类型：期刊论文

【摘要】：目的:观察氨基葡萄糖(Glucosamine,GLU)、骨碎补(Drynaria rhizome,DR)和虾青素(Astaxanthin,AST)单独和联合干预对大鼠急性骨关节炎的影响及其差别,为研发对骨关节炎有预防和改善作用的功能性食品提供理论依据。方法:120只雄性SD大鼠随机分为10组:空白对照组、模型对照组、阳性对照组和7个受试物组(GLU、DR、AST、GLU+DR、GLU+AST、DR+AST、GLU+DR+AST组),空白组和模型组灌胃给予蒸馏水(10 m L/kg),阳性对照组灌胃给予双氯芬酸钠(2 mg/kg),GLU、DR、AST的灌胃剂量分别为:1000、250、120 mg/kg。采用关节腔注射白陶土与λ-鹿角菜胶诱发大鼠关节炎,在实验后的第1、3、5、7 d测量大鼠关节肿胀度,第7 d处死动物,取膝关节制作石蜡组织切片,观察关节软骨组织学病理变化,评价蛋白聚糖的减少程度,取滑膜用ELISA测定大鼠滑膜组织中IL-1β和TNF-α的水平。结果:与模型对照组相比,各受试物组大鼠膝关节肿胀度均有不同程度减小,其中变化较显著的是GLU、DR、GLU+DR组(p0.01);各受试物组组织病理学评分、滑膜组织中TNF-α和IL-1β含量均有不同程度的降低,其中变化较显著的是GLU+DR、GLU+DR+AST、GLU、DR组(p0.01)。结论:氨基葡萄糖、骨碎补对大鼠急性关节炎具有预防和保护作用,联合作用效果更好,而虾青素的作用效果相对差些。
[Abstract]:Objective: to observe the effects of Glucosamine Glu, Drynaria rhizome-DRN and astaxanthinus astaxanthin on acute osteoarthritis in rats. Methods 120 male Sprague-Dawley rats were randomly divided into 10 groups: blank control group and model control group. In the positive control group and the 7 subjects group, GLU DRX GLU ASTN Dr ASTU Dr ASTU Dr AST group, blank group and model group were given 10 mL / kg distilled water, and the positive control group were given diclofenac sodium 2 mg / kg GLU DRAST by intragastric perfusion, respectively. The dose was 250120 mg / kg / kg by articular cavity injection in the positive control group (n = 2 mg / kg GLU DRAST), and the oral dose of diclofenac sodium 2 mg / kg GLU DRAST in the positive control group was 250120 mg / kg / kg respectively by intraarticular injection of diclofenac sodium 2 mg / kg in the positive control group. Arthritic rats were induced by white clay and 位 -deer carrageenan. The degree of joint swelling was measured on the 7th day after the experiment, and the rats were killed on the 7th day. The knee joints were taken to make paraffin sections, and the pathological changes of articular cartilage were observed to evaluate the degree of decrease of proteoglycan. The levels of IL-1 尾 and TNF- 伪 in synovial tissue of rats were measured by ELISA. Results: compared with the model control group, the degree of knee joint swelling in each group was decreased in varying degrees. The histopathological score and the contents of TNF- 伪 and IL-1 尾 in synovial tissue of all subjects were decreased to some extent, among which the change was more significant in GLU Dr ASTASTU GLU Dr group (P 0.01). Conclusion: glucosamine, glucosamine, glutamate, glucosamine, glucosamine, glucosamine, glucosamine, glucosamine, glucosamine, glucosamine, glutamate, glucosamine, glucosamine, glucosamine, glucosamine, glucosamine, glucosamine, glucosamine, BMD has preventive and protective effects on acute arthritis in rats. The combined effect is better than astaxanthin.
【作者单位】：北京联合大学功能食品科学技术研究院;北京联合大学应用文理学院生物活性物质与功能食品北京市重点实验室;北京绿色金可生物技术股份有限公司;
【基金】：北京联合大学生物活性物质与功能食品北京市重点实验室开放课题(Zk70201501)
【分类号】：R151

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