砷联合MC染毒致小鼠肝损伤的效应研究

发布时间：2018-03-19 10:37

本文选题：砷　切入点：微囊藻毒素　出处：《毒理学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的观察砷(As)和微囊藻毒素(MC)单独及联合染毒对小鼠血清及肝组织肝功能指标和氧化损伤指标的影响。方法 40只健康KM小鼠随机分为4组,分别为对照组、染砷组(12.5μg/L)、染MC组(0.25μg/kg·dl)和联合染毒剂量组(12.5μg/L+0.25μg/kg·dl)。腹腔注射染毒10周,测定血清和肝组织中ALT、AST、MDA、SOD和GSH-Px水平。结果各染毒组血清白蛋白和ALT的水平与对照组比较差异均无统计学意义(P0.05);与对照组比较,各染毒组的AST水平均升高,其中染MC组和联合染毒组差异有统计学意义(P0.05);与对照组相比,染砷组和联合染毒组SOD和GSH-Px活性均增高,差异有统计学意义(P0.05)。MDA变化不明显。与对照组比较,肝组织中的白蛋白降低,变化有统计学意义(P0.05);与对照组比较染MC组和联合染毒组的ALT、AST水平均有升高,且差异有统计学意义(P0.05),各组间MDA含量差异无统计学意义(P0.05),各组间SOD活性呈下降趋势,但差异无统计学意义(P0.05);GSH-Px活性在各染毒组均显著增高,有统计学意义(P0.05)。肝组织DNA在染砷及染MC组时有损伤,联合染毒时损伤更明显。结论低剂量MC-LR及砷和MC联合染毒可以诱导小鼠肝功能损伤和DNA损伤,但抗氧化酶在染毒组增高,可对抗其氧化损伤作用。
[Abstract]:Objective to observe the effects of arsenic As) and microcystin MCV on liver function and oxidative damage in serum and liver tissue of mice. Methods 40 healthy km mice were randomly divided into four groups, control group. The arsenic group (12.5 渭 g / L), the MC group (0.25 渭 g / kg 路dl) and the combined dose group (12.5 渭 g / L 0.25 渭 g / kg 路dll) were injected intraperitoneally for 10 weeks. Sod and GSH-Px levels in serum and liver tissue were measured. Results the levels of serum albumin and ALT in each exposed group were not significantly different from those in the control group (P 0.05), and the AST levels in each exposure group were higher than those in the control group. Compared with the control group, the activities of SOD and GSH-Px in the arsenic exposed group and the combined exposure group were increased, and the difference was not significant in the MC group and the combined exposure group, and the difference was not significant compared with the control group. Compared with the control group, the level of alt AST in MC group and combined group was increased. The difference was statistically significant (P 0.05). There was no significant difference in MDA content among the groups. The activity of SOD decreased in each group, but the activity of GSH-Px increased significantly in all groups. DNA in liver tissue was damaged in arsenic and MC groups, especially in combined exposure. Conclusion low dose MC-LR and combined arsenic and MC could induce liver function damage and DNA damage in mice, but antioxidant enzymes were increased in the exposed group. It can resist its oxidative damage.
【作者单位】：赣南医学院预防医学系;
【基金】：国家自然科学基金(81460504,81260434,81060241) 江西省自然科学基金(20132BAB205071,20114BAB205041) 江西省教育厅科学技术研究(GJJ13689,GJJ14699) 江西省卫生厅科技计划(20113133)
【分类号】：R114

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