低水平职业性苯接触人群健康效应及生物标志物研究

发布时间：2018-03-20 03:15

本文选题：苯　切入点：健康效应　出处：《中国职业医学》2015年06期 　论文类型：期刊论文

【摘要】：目的探讨低水平职业性苯接触人群的健康效应,初步筛选效应生物标志物。方法采用整群抽样方法,以某石油化工厂532名低水平职业性苯接触工人[工作场所空气中苯的时间加权平均浓度(CTWA)中位数(M)低于3.25 mg/m3]为接触组,根据性别及年龄1∶1配对,选择同厂532名非苯作业人员为对照组。采用气相色谱法测定工作场所空气中苯的水平;通过健康检查比较2组人员的健康状况;采集2组人群周围静脉血,分离血清,采用酶联免疫吸附法测定血清中纤维蛋白溶酶原(PLG)和血小板碱性蛋白(PBP)水平,采用透射比浊法在全自动生化仪上测定载脂蛋白B100(Apo B100)水平。结果接触组人群工作场所空气中苯CTWA的M为0.04 mg/m3,对照组人群工作场所空气中未检出苯。接触组人群失眠、头晕和头痛检出率均高于对照组(6.95%vs 2.82%,5.64%vs2.63%,3.20%vs 0.94%,P0.05);接触组人群红细胞计数和血红蛋白水平均高于对照组(M:5.01×1012/L vs4.83×1012/L,147 g/L vs 145 g/L,P0.05);接触组人群血清中天冬氨酸氨基转移酶、碱性磷酸酶、总胆汁酸和免疫球蛋白M水平均高于对照组(M:25 U/L vs 23 U/L,73 U/L vs 69 U/L,5.80μmol/L vs 5.45μmol/L,1.23 g/L vs1.15 g/L,P0.05)。接触组人群血清中PLG水平高于对照组(M:6.48μg/L vs 5.64μg/L,P0.05);多元线性回归分析结果显示,低水平苯接触对PLG水平有影响(P0.05)。接触组和对照组人群PBP和Apo B100水平比较,差异均无统计学意义(M:48.00 ng/L vs 44.00 ng/L,0.83 g/L vs 0.83 g/L,P0.05)。结论低水平职业性苯接触可对作业人群的神经系统、血液系统、肝功能和免疫功能产生一定影响;血清PLG水平可作为潜在的职业性低水平苯接触的效应生物标志物。
[Abstract]:Objective to investigate the health effects of low level occupational benzene exposure population and to screen the biomarkers for the effects. Methods Cluster sampling was used. In a petrochemical plant, 532 workers exposed to low-level occupational benzene [median concentration of benzene in the workplace air with a median concentration of CTWA less than 3.25 mg/m3] were matched according to sex and age of 1: 1. 532 non-benzene-exposed workers in the same factory were selected as control group. The level of benzene in workplace air was measured by gas chromatography; the health status of the two groups was compared by health examination; the peripheral venous blood was collected from two groups of people and the serum was separated. The levels of plasminogen plasminogen (PLG) and platelet basic protein (PBP) in serum were measured by enzyme linked immunosorbent assay (Elisa). The level of apolipoprotein B100 Apo B100 was determined by transmission turbidimetry. Results the M of benzene CTWA in workplace air of exposed group was 0.04 mg / m ~ (3), and no benzene was detected in workplace air of control group. The detection rate of dizziness and headache was higher than that of the control group (6.95 vs 2.82 vs 2.64 vs 2.63 vs 3.20 vs 0.94 P 0.05); the erythrocyte count and hemoglobin level of the exposed group were higher than that of the control group (M: 5.01 脳 10 12 / L vs4.83 脳 10 12 P / L 147g / L vs 145g / L P 0.05); the serum aspartate aminotransferase and alkaline phosphatase in the exposed group were higher than those in the control group. The levels of total bile acid and immunoglobulin M were higher than those of control group M: 25 U / L vs 23 U / L 73 U / L vs 69 U / L 5.80 渭 mol/L vs 5.45 渭 mol / L 1.23 渭 mol / L vs1.15 g / L P 0.050.The serum PLG level in the exposed group was higher than that in the control group (M: 6.48 渭 g / L vs 5.64 渭 g / L P 0.05). There was no significant difference in PBP and Apo B100 levels between the exposed group and the control group. There was no significant difference in the levels of PBP and Apo B100 between the exposed group and the control group. Conclusion there was no significant difference in the levels of PBP and Apo B100 between the exposed group and the control group. Conclusion there is no significant difference in the levels of PBP and Apo B100 between the exposed group and the control group. Conclusion there is no significant difference in the levels of PBP and Apo B100 between the exposed group and the control group. The level of serum PLG may be used as a potential biomarker of occupational low level benzene exposure.
【作者单位】：中山大学公共卫生学院;广东省职业病防治院广东省职业病防治重点实验室;广东省妇幼保健院;
【基金】：国家科技支撑计划(2014BAI12B01) 国家自然科学基金重点项目(81430079) 广东省职业病防治重点实验室(2012A061400007) 广东省化学中毒与核辐射突发事件医学救援应急技术研究中心(2012A032100002) 广东省科技厅公益研究与能力建设专项基金(2014A020212249)
【分类号】：R135.12

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