经饮水甲基汞对小鼠脾脏中成熟免疫细胞影响的时间-效应特征

发布时间：2018-03-20 23:31

本文选题：甲基汞　切入点：脾脏　出处：《环境与职业医学》2017年03期 　论文类型：期刊论文

【摘要】：[目的]观察甲基汞暴露后不同时间点B10.S小鼠脾脏中髓系细胞、淋巴系细胞和骨髓中造血祖细胞生成相应成熟髓系细胞集落的数量及其动态变化,解释成熟免疫细胞动态变化的可能原因,探讨甲基汞引起机体免疫紊乱的可能机制。[方法]以6到8周龄的B10.S雌性小鼠为研究对象,随机分为对照组和实验组,分别饮用去离子水、1.25μmol/L甲基汞水溶液4周,测汞仪测定脾脏和脑中汞的含量,每周观察饮水和饮食消耗量。分别在第1周、2周和4周用动物电子称称量小鼠体重,并且在第1周、2周和4周用流式细胞仪检测小鼠脾脏中巨噬细胞、单核细胞、中性粒细胞、B淋巴细胞、CD4~+T细胞、CD8~+T细胞和自然杀伤(NK)细胞的数量,并用细胞集落形成试验(CFU,colony-formation units)检测骨髓造血祖细胞生成成熟髓系细胞的能力。[结果]2组小鼠饮水量、饮食消耗量、体重变化量之间的差异均无统计学意义(P0.05)。饮用甲基汞溶液1周后,与对照组相比,小鼠脾脏中单核细胞、中性粒细胞在脾脏细胞中的百分比增高;第2周,单核细胞、巨噬细胞、中性粒细胞的数量百分比降低(P0.05)。与对照组相比,B细胞数量百分比在第2周和第4周增高(P0.05),CD4~+T细胞的数量百分比在第4周下降,CD8~+T细胞、NK细胞的数量百分比在第2周下降,差异均具有统计学意义(P0.05)。CFU实验表明,在饮用甲基汞后第1周,粒细胞-红细胞-巨噬细胞-单核细胞集落(GEMM)数量、粒细胞-巨噬细胞集落(GM)数量、粒细胞集落(G)和巨噬细胞集落(M)数量增多,与对照组相比,差异有统计学意义(P0.05);与对照组相比,饮用甲基汞2周后,GEMM和GM数量下降(P0.05),4周后G和M数量增多(P0.05)。[结论]甲基汞可导致小鼠骨髓中髓系祖细胞分化生成相应成熟细胞的能力先增强后减弱再恢复至正常水平,进而导致单核细胞、中性粒细胞、巨噬细胞的百分比呈现先升高后降低再逐渐恢复的趋势,CD4~+T细胞占脾脏细胞的百分比总体呈现下调的趋势,CD8~+T细胞、NK细胞占脾脏细胞的百分比先下调后上升,B细胞占脾脏细胞的比例总体呈现上调的趋势。甲基汞暴露后不同时间点小鼠脾脏中成熟免疫细胞的动态变化很复杂,总体上可以用CFU的变化来解释髓系细胞变化的原因。成熟免疫细胞的数量百分比随染毒时间的变化而发生改变,提示甲基汞引起的免疫紊乱可能与其导致的成熟免疫细胞比例失调有关。
[Abstract]:[objective] to observe the number and dynamic changes of myeloid cells in spleen, lymphoid cells and hematopoietic progenitor cells in bone marrow of B10.S mice at different time points after exposure to methylmercury. To explain the possible causes of dynamic changes of mature immune cells and to explore the possible mechanism of immune disorder induced by methylmercury. [methods] female B10.S mice aged 6 to 8 weeks were randomly divided into two groups: control group and experimental group. After drinking deionized water of 1. 25 渭 mol/L methylmercury solution for 4 weeks, the contents of mercury in spleen and brain were measured, and the consumption of drinking water and diet were observed weekly. The number of macrophages, monocytes, neutrophil B lymphocytes, CD8T cells and natural killer NK cells in the spleen of mice were detected by flow cytometry at week 1, week 2 and week 4, respectively. The ability of bone marrow hematopoietic progenitor cells to produce mature myeloid cells was detected by CFU colony-formation test. [results] the drinking water and diet consumption of the two groups were measured. After drinking methylmercury solution for 1 week, the percentage of monocytes and neutrophils in spleen of mice was higher than that of control group. Macrophages, Compared with the control group, the percentage of neutrophils increased at the 2nd and 4th week. The percentage of CD8- T cells decreased at the 4th week, and the percentage of NK cells decreased at the second week. The difference was statistically significant (P 0.05). CFU test showed that the number of granulocyte-erythrocyte macrophage-monocyte colony (GEMMM), granulocyte-macrophage colony (GMCs) and granulocyte-macrophage colony (GMCs) were observed at the first week after drinking methylmercury. The number of granulocyte colony (G) and macrophage colony (M) increased, compared with the control group, the difference was statistically significant (P 0.05), compared with the control group, After 2 weeks of exposure to methylmercury, the number of GEMM and GM decreased and the number of G and M increased after 4 weeks. [conclusion] methylmercury can induce the ability of bone marrow progenitor cells to differentiate and produce corresponding mature cells in mice bone marrow at first, then weaken and then return to normal level. Which in turn leads to monocytes, neutrophils, The percentage of macrophages increased first, then decreased and then recovered gradually. The percentage of CD4T cells in spleen cells was down-regulated, and the percentage of NK cells in spleen cells decreased first and then increased. The percentage of spleen cells in mice showed an upward trend. The dynamic changes of mature immune cells in mice spleen were complicated at different time points after methylmercury exposure. In general, the changes in CFU can be used to explain the changes in myeloid cells. The percentage of mature immune cells changes with the time of exposure. The results suggest that the immune disorder caused by methylmercury may be related to the imbalance of mature immune cells.
【作者单位】：复旦大学公共卫生学院教育部公共卫生重点实验室;
【基金】：国家自然科学基金青年基金资助项目(编号:81402707)
【分类号】：R114

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