胶体金免疫层析法应用于环境水体中霍乱检测的效果评价

发布时间：2018-03-21 14:26

本文选题：胶体金免疫层析法　切入点：传统分离培养　出处：《现代预防医学》2015年03期 　论文类型：期刊论文

【摘要】：目的验证胶体金免疫层析法应用于环境水样本中霍乱检测的特异性、灵敏性和实用性,寻求简便、可靠的环境水中霍乱检测方法。方法环境水样置36.5℃经6 h增菌培养后,用胶体金免疫层析法检测,同时用传统分离培养法,培养后的菌落用血清玻片凝集方法作为鉴别霍乱弧菌的金标准,且用统计学方法对这两种方法检测的结果进行比较。结果 747份水样中胶体金试验阳性19份,分离培养法培养出16株霍乱弧菌,经χ2检验,差异无统计学意义(χ2=0.61,P0.05),表明胶体金免疫层析法与传统分离培养法检测外环境水中霍乱的结果之间差异无统计学意义。胶体金免疫层析法检测外环境水中霍乱的灵敏度为100.00%,特异性为99.59%,假阳性率为0.41%,假阴性率为0.00%,与传统分离培养法的结果一致率为99.60%。结论胶体金免疫层析法检测外环境水中霍乱的敏感度好(100.00%),特异性高(99.59%),与传统分离培养法的结果一致性较高,对外环境水中霍乱检测具有可靠性、简便性、实用性,适宜在基层作为辅助检测方法推广应用。
[Abstract]:Objective to verify the specificity, sensitivity and practicability of colloidal gold immunochromatography for the detection of cholera in environmental water samples, and to find a simple and reliable method for the detection of cholera in environmental water. The colloidal gold immunochromatographic assay was used to identify Vibrio cholerae by using the traditional isolation and culture method. The cultured colony was identified by serum slide agglutination method, which was used as the gold standard for the identification of Vibrio cholerae. Results among 747 water samples, 19 were positive for colloidal gold test. 16 strains of Vibrio cholerae were isolated and cultured. There was no significant difference between colloidal gold immunochromatographic assay and traditional separation culture method in the detection of cholera in external environment water. Sensitivity of Colloidal Gold Immunochromatographic Assay for Cholera in Outer Environment Water. Conclusion the sensitivity of colloidal gold immunochromatographic assay for detecting cholera in external water is 100.000.The specificity is 99.59g, the false positive rate is 0.41and the false negative rate is 0.000.Conclusion the sensitivity of colloidal gold immunochromatography in detecting cholera in external environment water is good, and the specificity is high. The results of culture method were consistent. Cholera detection in external environmental water is reliable, simple and practical. It is suitable to be used as an auxiliary detection method in basic level.
【作者单位】：江西省疾病预防控制中心;
【分类号】：R123.1

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