纳米硒与亚硒酸钠在高碘致小鼠氧化损伤中的干预作用

发布时间：2018-03-22 09:32

本文选题：高碘　切入点：氧化性损伤　出处：《环境与职业医学》2015年01期 　论文类型：期刊论文

【摘要】：[目的]研究比较纳米硒和亚硒酸钠对高碘致小鼠部分组织氧化损伤的干预作用。[方法]将48只雄性昆明小鼠被随机分为适碘组[50μg/L碘酸钾(KIO3)]、高碘组(3 000μg/L KIO3)、亚硒酸钠组(3 000μg/L KIO3+0.193 mg/kg硒)、纳米硒组(3 000μg/L KIO3+0.193 mg/kg硒),每组12只。4周后处死小鼠,测定血清、肝脏、肾脏组织的谷胱甘肽过氧化物酶(GSH-Px)活力、以及丙二醛(MDA)水平。观察甲状腺病理变化。[结果]与适碘组相比,高碘组血清、肝脏和肾脏中GSH-Px活力均明显下降,差异有统计学意义(P0.05);肝脏中MDA含量下降,差异有统计学意义(P0.05);肝脏和肾脏脏器系数均增加,差异有统计学意义(P0.05)。与高碘组相比,亚硒酸钠组血清和肝脏中GSH-Px活力均明显升高,差异有统计学意义(P0.05);肾脏中MDA含量增加,差异有统计学意义(P0.05);肝脏脏器系数下降,差异有统计学意义(P0.05);血清和肝脏中MDA含量均下降。纳米硒组血清、肝脏和肾脏中GSH-Px活力均明显增加,差异有统计学意义(P0.05);血清和肝脏中MDA含量均明显下降,在肾脏中增加,差异有统计学意义(P0.05);肾脏脏器系数下降,差异有统计学意义(P0.05)。高碘组甲状腺组织出现弥漫胶质性甲状腺肿。[结论]高碘摄入可致小鼠氧化性损伤,补硒有一定干预作用,纳米硒的干预效果优于亚硒酸钠。
[Abstract]:[objective] to study the effects of nano selenium and sodium selenite on oxidative damage induced by iodine in mice. [methods] 48 male Kunming mice were randomly divided into two groups: iodine group [50 渭 g / L potassium iodate KIO _ 3], high iodine group (n = 3 000 渭 g / L KIO _ 3), selenite group (n = 30). Sodium group (3 000 渭 g / L KIO3 0.193 mg/kg) and nanometer selenium group (3 000 渭 g / L KIO3 0.193 mg/kg selenide) were killed in each group after 4 weeks. The activity of glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in serum, liver and kidney were measured. The difference was statistically significant (P 0.05), the content of MDA in liver was decreased (P 0.05), the coefficient of liver and kidney organs was increased, and the difference was statistically significant (P 0.05). Compared with the high iodine group, the activity of GSH-Px in serum and liver of sodium selenite group was significantly higher than that of high iodine group. The difference is statistically significant (P 0.05); the content of MDA in kidney is increased, the difference is significant (P 0.05); the coefficient of liver organ is decreased, the difference is statistically significant (P 0.05); the content of MDA in serum and liver is decreased. The activity of GSH-Px in liver and kidney was significantly increased, the difference was statistically significant (P 0.05), the content of MDA in serum and liver was significantly decreased, and increased in kidney, the difference was statistically significant (P 0.05), and the organ coefficient of kidney was decreased. The difference was statistically significant (P 0.05). There was diffuse glial goiter in thyroid tissue in high iodine group. [conclusion] excessive iodine intake can induce oxidative injury in mice, and selenium supplementation has a certain intervention effect, and the intervention effect of nanometer selenium is better than that of sodium selenite.
【作者单位】：沈阳医学院公共卫生学院环境卫生学教研室;沈阳医学院基础医学院病理学教研室;
【基金】：沈阳医学院科学研究基金(编号:20101019)
【分类号】：R151.2

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