河北省石家庄市手足口病流行中健康人肠道病毒带毒情况及病原分析

发布时间：2018-03-23 06:30

本文选题：手足口病(HFMD)　切入点：健康儿童　出处：《病毒学报》2017年03期 　论文类型：期刊论文

【摘要】：为了解河北省石家庄市健康儿童中肠道病毒带毒情况及血清型构成,对2010~2012年石家庄市的3个县区1 223例0~6岁的健康儿童及406名监护人(健康成人)采集咽拭子和粪便标本进行核酸检测鉴定肠道病毒血清型,并进行分子流行病学分析,为肠道病毒感染相关疾病的防控和治疗提供基础资料。1 223名健康儿童的标本检测结果中,肠道病毒(Enterovirus,EV)阳性的283例,阳性率为23.14%(283/1 223)。其中,以非肠道病毒71型非柯萨奇病毒A组16型的EV(Other-Enterovirus,other-EVs)阳性为主,占EV阳性的62%(175/283),而引起手足口病常见的EV病原体肠道病毒71型(Enterovirus A71,EV71)和柯萨奇病毒A组16型(Coxsackievirus A16,CVA16)阳性率较低,分别占19%(55/283)和18%(51/283);将结果为other-EVs阳性的175名健康儿童的标本全部进行病毒分离鉴定,共分离到25株病毒,分离率为14.29%(25/175),其中柯萨奇病毒B组5型(Coxsackievirus B5,CVB5)分离率最高,为40%(10/25)、柯萨奇病毒B组13型(Coxsackievirus B3,CVB3)分离率为24%(6/25)、埃可病毒21型(Enteric cytopathic human orphan virus 21,ECHO21)分离率为16%(4/25)、其他EV株分离率为20%(5/25)。提示,应加强对CVB3和CVB5等other-EVs的监测。
[Abstract]:In order to understand the situation of enterovirus infection and serotype composition in healthy children in Shijiazhuang City Hebei Province. From 2010 to 2012, 1 223 healthy children aged 0 to 6 years old and 406 guardians (healthy adults) were collected from 3 counties of Shijiazhuang City to identify enterovirus serotype by nucleic acid detection and molecular epidemiology analysis. To provide basic data for the prevention, control and treatment of enterovirus infection related diseases, 283 cases (23.14%) were positive in Enterovirus-EV (23.14%), among which, the positive rate was 23.14%. The positive rate of EVX Other-Enterovirus-other-EVs16 in group A of non-coxsackie virus 71 (non-Coxsackievirus 71) and Coxsackievirus A16 (Coxsackievirus A16 CVA16) in group A was lower than that in group A (62175 / 283%), while enterovirus A71 (Enterovirus A71EV71) and Coxsackievirus A16 (CVA16), the common pathogens causing HFMD, were lower than that of Coxsackievirus A16 (CVA16). The samples of 175 healthy children who were positive for other-EVs were isolated and identified, and 25 strains were isolated, the isolation rate was 14.29% 25 / 175%, and the isolation rate of Coxsackievirus B group 5 was the highest, among which Coxsackievirus B5 CVB5 was the highest. Coxsackievirus type 13 (Coxsackievirus B3CVB3) of Coxsackievirus B was 24 / 25, Enteric human orphan virus 21 / ECHO21 was 16 / 25, and other EV strains were 2055 / 25, suggesting that the monitoring of other-EVs such as CVB3 and CVB5 should be strengthened.
【作者单位】：河北省石家庄市疾病预防控制中心;中国疾病预防控制中心病毒病预防控制所;
【分类号】：R181.3;R512.5

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