黑芝麻油对环磷酰胺诱导大鼠股骨骨丢失的影响

发布时间：2018-03-23 16:17

本文选题：环磷酰胺　切入点：大鼠　出处：《营养学报》2015年04期

【摘要】：目的应用骨生物力学和micro-CT技术,探讨黑芝麻油对环磷酰胺诱导的大鼠骨丢失模型中股骨的显微结构和生物力学的影响,并与阳性药物阿仑膦酸钠对比。方法 32只SPF级雄性SD大鼠,随机分为正常对照组(CON)、环磷酰胺模型组(CP)、阿仑膦酸钠治疗组(ALD)、黑芝麻油治疗组(BSO),连续干预15d。实验结束后,取右侧股骨进行生物力学检测,然后进行Micro-CT扫描及三维重建。结果与CON组比较,CP组大鼠股骨的骨微观参数:骨体积分数、骨小梁数量、骨小梁厚度和骨密度明显减少,骨小梁分离度和结构模型指数明显增高;而股骨的骨生物力学参数:最大强度、断裂强度、断裂应变及韧性系数明显减少,刚性系数明显增加。与CP组相比,ALD组大鼠股骨的骨微观参数均得到了良好地修复,但骨生物力学参数中仅断裂应变、韧性系数和刚性系数三个参数得到了明显修复。而BSO组大鼠股骨的微观参数和生物力学参数均得到了显著修复。结论 3ml/kg·d的黑芝麻油能良好的对抗CP诱导的大鼠骨丢失模型中股骨微观结构和骨质量的退化,提示黑芝麻油的良好抗骨质疏松作用前景。
[Abstract]:Objective to investigate the effects of black sesame oil on the microstructure and biomechanics of femur in cyclophosphamide induced bone loss model in rats using bone biomechanics and micro-CT techniques. Methods 32 SPF male SD rats were randomly divided into normal control group, cyclophosphamide model group, alendronate treatment group and black sesame oil treatment group for 15 days. Biomechanical examination was performed on the right femur, and then Micro-CT scanning and 3D reconstruction were performed. Results compared with the CON group, the bone microscopic parameters of the CP group: bone volume fraction, bone trabecular number, trabecular thickness and bone density decreased significantly. The bone trabecular separation degree and structural model index were significantly increased, while the bone biomechanical parameters of femur: maximum strength, fracture strength, fracture strain and toughness coefficient were significantly decreased. Compared with CP group, the bone microcosmic parameters of ALD group were repaired well, but only fracture strain was found in bone biomechanical parameters. The three parameters of toughness coefficient and rigidity coefficient were obviously repaired, while the microcosmic and biomechanical parameters of femur in BSO group were significantly repaired. Conclusion the black sesame oil of 3ml/kg d can resist CP induced rats well. Degeneration of femur microstructure and bone mass in bone loss model. The results suggest that black sesame oil has a good anti-osteoporosis effect.
【作者单位】：广东医学院药学院;
【基金】：国家自然科学基金资助项目(No.81273518) 湛江市科技计划项目(No.2012C3104017)
【分类号】：R151

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