宫内节育器表面的细菌生物膜研究

发布时间：2018-03-25 16:38

本文选题：宫内节育器　切入点：盆腔炎性疾病　出处：《中国工程科学》2015年06期

【摘要】：目的:宫内节育器(IUD)是目前常用的长效避孕措施之一,然而众多研究认为IUD的应用增加了盆腔炎性疾病(PID)的发生率。近年研究发现细菌生物膜(BF)与感染性疾病的发生息息相关,本研究拟探究无盆腔炎疾病妇女的不同类型宫内节育器表面是否存在BF,了解IUD是否为BF形成提供平台,是否增加盆腔炎性疾病的易感性。方法:不同类型IUD标本,根据表面清洁程度分别进行单独电子扫描电镜(SEM)观察,单独需氧、厌氧细菌培养,以及SEM观察+培养了解IUD表面是否存在BF。结果:共纳入IUD标本86例,85例非PID女性的IUD,同期1例盆腔感染性疾病患者的IUD。25例进行单独SEM观察,阳性1例,其余标本未见典型BF结构。单独细菌培养47例,需氧细菌培养阳性1例,见大面积菌苔覆盖,考虑污染可能性大,其余标本未见细菌生长。SEM联合培养14例,细菌培养阳性,电镜无阳性发现。其余培养及SEM观察阴性。结论:不论IUD的类型如何,非PID女性体内的IUD表面无BF存在,不同与其他体内医疗装置,BF的形成不能成为限制IUD应用的理由。
[Abstract]:Objective: IUD is one of the commonly used methods of long-term contraception. However, many studies suggest that the use of IUD increases the incidence of pelvic inflammatory diseases. Recent studies have found that bacterial biofilm is closely related to the occurrence of infectious diseases. This study was designed to investigate the presence of BFs on the surface of different types of intrauterine devices in women without pelvic inflammatory disease, to understand whether IUD provides a platform for BF formation and increases susceptibility to pelvic inflammatory diseases. Methods: different types of IUD specimens, According to the degree of surface cleanness, the single electron scanning electron microscope (SEM) observation was carried out, and the aerobic and anaerobic bacteria were cultured separately. Results: 86 IUD specimens were included in 85 cases of non-#en3# women, and 1 case of IUD.25 of pelvic infectious disease were observed by SEM alone, and 1 case was positive in the same period. No typical BF structure was found in the other specimens. There were 47 cases of bacterial culture alone, 1 case of aerobic bacteria culture positive, covered with large area of bacterial coating, considering the possibility of contamination, 14 cases of bacteria growth. SEM co-culture was not found in other specimens, the bacteria culture was positive. No positive results were found in electron microscope. Other cultures and SEM observation were negative. Conclusion: no matter the type of IUD, there is no BF on the surface of IUD in non-#en2# women. Different from the formation of BF in other medical devices in vivo, it can not be a reason to restrict the application of IUD.
【作者单位】：四川大学华西第二医院;
【基金】：宫内节育器表面生物膜及细菌粘附机制(0040215401128)
【分类号】：R169.4

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