镉致大鼠血液、肝脏、肾脏和睾丸的毒性损伤

发布时间：2018-03-26 17:36

本文选题：镉　切入点：大鼠　出处：《毒理学杂志》2017年01期

【摘要】：目的为研究镉对大鼠血液、肝脏、肾脏和睾丸的毒性损伤。12只SD大鼠分为对照组和镉处理组,每组6只,对照组自由饮用自来水,用0.9%的生理盐水腹腔注射,镉处理组用无菌生理盐水配制的Cd Cl2溶液腹腔注射,剂量为2 mg/kg·bw,注射容量均为10 ml/kg·bw,1次/d,共5周。实验结束,剖杀大鼠,采集血液,检测大鼠WBC、Hb、RBC、MCH、MCV、HCT和MCHC等血液参数;取大鼠肝脏、肾脏和睾丸并称重,计算脏器系数;匀浆组织,检测组织内MDA、GSH、GSH-PX、CAT和SOD含量。结果表明,与对照组相比,镉处理组大鼠RBC升高差异有统计学意义(P0.01),Hb、MCH、MCV和HCT均降低差异有统计学意义(P0.05或P0.01),MCHC差异则无统计学意义。镉组大鼠肝脏和肾脏的脏器系数升高(P0.05或P0.01),但大鼠睾丸的脏器系数与对照组比较降低(P0.01)。镉组肝脏、肾脏和睾丸中MDA均升高(P0.05或P0.01);肝脏、肾脏中GSH升高(P0.01),GSH-PX、SOD和CAT活性(P0.05或P0.01),但睾丸中这些指标与之相反,GSH含量降低(P0.01);GSH-PX、SOD和CAT活性升高(P0.05或P0.01),上述差异均有统计学意义。实验结果表明,镉可引起大鼠血液、肝脏、肾脏和睾丸的毒性损伤。
[Abstract]:Objective to study the toxic effects of cadmium on blood, liver, kidney and testis of rats. Twelve SD rats were divided into two groups: control group (n = 6) and cadmium treated group (n = 6). The control group drank tap water freely and was injected intraperitoneally with 0.9% normal saline. The cadmium treated group was injected intraperitoneally with aseptic saline solution of CD Cl2, the dose was 2 mg/kg Bww, the injection volume was 10 ml/kg BWN once a day for 5 weeks. At the end of the experiment, the rats were killed, the blood was collected, and the blood parameters such as Cl2 and MCHC were detected, and the liver of the rats was taken out. Kidney and testis were weighed, organ coefficient was calculated, and homogenate tissue was used to detect the contents of GSH-PXX cat and SOD in tissue. The results showed that, compared with the control group, the contents of GSH-PXX cat and SOD in the homogenate tissue were measured. There was significant difference in the increase of RBC in the cadmium treatment group. There was no significant difference in the decrease of MCHV and HCT between the two groups. However, the visceral coefficient of liver and kidney in the cadmium group was higher than that in the control group (P0.05 or P0.01), but the visceral organs in the testis of the rats were higher than that of the control group (P 0.05 or P 0.01), but the visceral organs of the testis of the rats in the cadmium group were significantly higher than those in the control group. Compared with the control group, the coefficient of apparatus was decreased by P0.010.The liver of cadmium group was lower than that of control group. MDA activity in kidney and testis was increased by P0.05 or P0.01G, and GSH activity in liver and kidney was increased by P0.01G or P0.01P, but the contents of GSH-PXG and CAT in testis were significantly lower than those in testis (P 0.05 or P0.01g). The results showed that there were significant differences between the two groups, and the results showed that the contents of GSH-PXO and GSH-PXG in the testis were significantly higher than those in the testes (P0.05 or P0.01g / L). The results showed that there were significant differences between the two groups. Cadmium can cause toxic damage to blood, liver, kidney and testis in rats.
【作者单位】：河南科技大学动物科技学院;河南科技大学校医院;
【基金】：河南科技大学博士科研启动基金(4025-13480037)、河南科技大学青年科学基金(4025-13350068)
【分类号】：R114

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